狼疮肾炎患者调节性 T 细胞激活频率增加。

Increased frequency of activated regulatory T cells in patients with lupus nephritis.

机构信息

Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Department of Immunology, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

出版信息

Hum Immunol. 2022 Jul;83(7):574-579. doi: 10.1016/j.humimm.2022.05.001. Epub 2022 May 12.

DOI:10.1016/j.humimm.2022.05.001

PMID:35568620

Abstract

BACKGROUND AND OBJECTIVE

Lupus nephritis (LN) is one of the common manifestations of systemic lupus erythematosus (SLE), affecting the quality of life of patients. Abnormality in the adaptive immune response, such as T cell response, plays the main role in the pathogenesis of SLE and LN. In this study, we aimed to evaluate the role of different subpopulations of regulatory T cells (Tregs) and effector T cells (Teff) in LN patients and compare them with SLE patients.

MATERIALS AND METHODS

A total of 48 participants were enrolled in this study and divided into 3 groups: (i) patients with SLE; (ii) patients with LN; and (iii) healthy controls (HCs). The frequencies of CD4 CD25 CD45RA Foxp3 activated Tregs (aTregs), CD4 CD25 CD45RA Foxp3 resting Tregs (rTregs), CD4 CD25 CD45RA Foxp3 non-Tregs, CD4 CD25 Foxp3 Teff, and Tregs were analyzed in all subjects using a flow cytometer.

RESULTS

LN patients had a significantly increased frequency of aTregs and Tregs compared with SLE patients (standardized mean difference [SMD] = 0.50; 95% CI [-0.26, 1.25]; p > 0.05 and SMD = 0.60; 95% CI [-0.16, 1.36]; p > 0.05, respectively). Patients with LN had a significantly increased frequency of Teff compared with SLE patients (SMD = 0.49; 95% CI [-0.26, 1.24]; p > 0.05). However, an increased ratio of Tregs/Teff was observed in LN patients compared with SLE patients (SMD = -0.25; 95% CI [-0.97, 0.48]; p > 0.05).

CONCLUSION

Patients with LN showed immunoregulatory properties, in which both aTregs and Tregs had increased frequencies.

摘要

背景和目的

狼疮肾炎（LN）是系统性红斑狼疮（SLE）的常见表现之一，影响患者的生活质量。适应性免疫反应异常，如 T 细胞反应，在 SLE 和 LN 的发病机制中起主要作用。在本研究中，我们旨在评估不同调节性 T 细胞（Treg）和效应 T 细胞（Teff）亚群在 LN 患者中的作用，并将其与 SLE 患者进行比较。

材料和方法

共纳入 48 名参与者，分为 3 组：（i）SLE 患者；（ii）LN 患者；和（iii）健康对照者（HCs）。使用流式细胞仪分析所有受试者中 CD4 CD25 CD45RA Foxp3 活化 Treg（aTreg）、CD4 CD25 CD45RA Foxp3 静息 Treg（rTreg）、CD4 CD25 CD45RA Foxp3 非 Treg、CD4 CD25 Foxp3 Teff 和 Treg 的频率。

结果

与 SLE 患者相比，LN 患者的 aTreg 和 Treg 频率显著增加（标准化均数差 [SMD]分别为 0.50；95%置信区间 [CI]：-0.26，1.25；p>0.05 和 SMD 分别为 0.60；95%CI：-0.16，1.36；p>0.05）。与 SLE 患者相比，LN 患者的 Teff 频率显著增加（SMD 分别为 0.49；95%CI：-0.26，1.24；p>0.05）。然而，与 SLE 患者相比，LN 患者的 Treg/Teff 比值增加（SMD 分别为-0.25；95%CI：-0.97，0.48；p>0.05）。