PEGASE, INRAE, Institut Agro, 35590, Saint-Gilles, France.
Sci Rep. 2022 May 14;12(1):7991. doi: 10.1038/s41598-022-12050-6.
The ability of pigs to cope with inflammatory challenges may by modified by selection for residual feed intake (RFI), a measure of feed efficiency. In the current study, we evaluated skeletal muscle metabolic responses to degraded hygiene conditions in pigs divergently selected for RFI. At 82 d of age, low RFI and high RFI pigs were housed in either poor or good hygiene conditions. After a 6-week challenge, the poor hygiene conditions induced a decrease in growth performance (P < 0.001) and in plasma IGF-I concentrations (P < 0.003) in both lines. In the slow-twitch oxidative semispinalis muscle, poor hygiene conditions induced a shift towards a more oxidative metabolism and an activation of the AMPK pathway in pigs of both RFI lines. In the fast-twitch glycolytic longississimus muscle, poor hygiene conditions were associated to a less glycolytic metabolism in the HRFI line only. Poor hygiene conditions also increased the protein level of lipidation of microtubule-associated protein 1 light-chain 3β (LC3-II) in both RFI lines, suggesting an activation of the autophagy pathway. Altogether, the data revealed muscle-type specific metabolic adaptations to poor hygiene conditions, which may be related to different strategies to fuel the activated immune system.
猪应对炎症挑战的能力可能会因选择残余采食量（RFI）而改变，RFI 是饲料效率的一种衡量标准。在本研究中，我们评估了在 RFI 差异选择下，猪对退化卫生条件的骨骼肌代谢反应。在 82 日龄时，低 RFI 和高 RFI 猪分别被安置在卫生条件较差或较好的环境中。经过 6 周的挑战后，较差的卫生条件导致了两条品系的生长性能（P < 0.001）和血浆 IGF-I 浓度（P < 0.003）均下降。在慢收缩氧化型半棘肌中，较差的卫生条件导致两条 RFI 线的猪的代谢向更氧化的方向转变，并激活了 AMPK 途径。在快收缩糖酵解型最长肌中，较差的卫生条件仅与 HRFI 线的糖酵解代谢减少有关。较差的卫生条件还增加了两条 RFI 线中微管相关蛋白 1 轻链 3β（LC3-II）的脂质化蛋白水平，表明自噬途径被激活。总之，这些数据揭示了肌肉类型对较差卫生条件的特定代谢适应，这可能与激活的免疫系统的不同供能策略有关。
