NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), National University of Singapore, Singapore 117456, Singapore; Synthetic Biology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456, Singapore; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore; Wilmar-NUS Corporate Laboratory (WIL@NUS), National University of Singapore, Singapore 117599, Singapore.
NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), National University of Singapore, Singapore 117456, Singapore; Synthetic Biology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456, Singapore; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore; Wilmar-NUS Corporate Laboratory (WIL@NUS), National University of Singapore, Singapore 117599, Singapore; HH@NUS SINERGY Laboratory, National University of Singapore, Singapore 117544, Singapore.
Curr Opin Biotechnol. 2022 Aug;76:102731. doi: 10.1016/j.copbio.2022.102731. Epub 2022 May 12.
Biosensors could enable a wide range of applications in environmental monitoring, pathogen detection, and biomarker diagnostics. While conventional diagnostic platforms like chemical sensors and PCR-based assays are capable of highly sensitive detection in laboratory conditions, their configurations, production cost, and operational requirements are not suitable for applications beyond the laboratory. Recent advances in synthetic biology, bioelectronics, and materials sciences have paved the way for the creation of novel microbial biosensing devices, which integrate core synthetic biosensors with state-of-the-art deployment platforms to create unique biosensor products. Here, we review the latest developments in microbial biosensing devices and discuss challenges and perspectives towards realizing their broad applications in real-world settings.
生物传感器可以在环境监测、病原体检测和生物标志物诊断等领域实现广泛的应用。虽然传统的诊断平台，如化学传感器和基于 PCR 的检测方法，能够在实验室条件下进行高灵敏度的检测，但它们的配置、生产成本和操作要求并不适合实验室以外的应用。最近，合成生物学、生物电子学和材料科学的进展为新型微生物生物传感设备的创造铺平了道路，这些设备将核心合成生物传感器与最先进的部署平台集成在一起，创造出独特的生物传感器产品。在这里，我们回顾了微生物生物传感设备的最新发展，并讨论了在现实环境中实现其广泛应用所面临的挑战和前景。
