Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
Biotechnol Adv. 2023 Jan-Feb;62:108077. doi: 10.1016/j.biotechadv.2022.108077. Epub 2022 Dec 9.
Genetically encoded biosensors are the vital components of synthetic biology and metabolic engineering, as they are regarded as powerful devices for the dynamic control of genotype metabolism and evolution/screening of desirable phenotypes. This review summarized the recent advances in the construction and applications of different genetically encoded biosensors, including fluorescent protein-based biosensors, nucleic acid-based biosensors, allosteric transcription factor-based biosensors and two-component system-based biosensors. First, the construction frameworks of these biosensors were outlined. Then, the recent progress of biosensor applications in creating versatile microbial cell factories for the bioproduction of high-value chemicals was summarized. Finally, the challenges and prospects for constructing robust and sophisticated biosensors were discussed. This review provided theoretical guidance for constructing genetically encoded biosensors to create desirable microbial cell factories for sustainable bioproduction.
基因编码生物传感器是合成生物学和代谢工程的重要组成部分，因为它们被视为动态控制基因型代谢和筛选理想表型的强大工具。本综述总结了不同基因编码生物传感器的构建和应用的最新进展，包括荧光蛋白生物传感器、核酸生物传感器、变构转录因子生物传感器和双组分系统生物传感器。首先，概述了这些生物传感器的构建框架。然后，总结了生物传感器在创建多功能微生物细胞工厂以生物生产高价值化学品方面的最新进展。最后，讨论了构建稳健和复杂的生物传感器的挑战和前景。本综述为构建基因编码生物传感器以创建理想的微生物细胞工厂进行可持续生物生产提供了理论指导。
