Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Egypt.
Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Egypt.
Toxicology. 2022 May 15;473:153208. doi: 10.1016/j.tox.2022.153208. Epub 2022 May 13.
With recent progress in the manufacture and applications of nickel oxide nanoparticles (NiO NPs), concerns about their adverse effects are increasing. Hesperidin (HSP) is a citrus flavonoid that has a potent anti-inflammatory, antioxidant and free radical scavenging activities. This study aims to investigate the protective effect of HSP against testicular and spermatological damages induced by NiO NPs in male rats. Forty rats were randomly and equally divided into four groups: control, NiO NPs, HSP and NiO NPs + HSP. NiO NPs (100 mg/kg) and/or HSP (100 mg/kg) were given daily by gavage for 60 days. Exposure to NiO NPs induced marked reproductive toxicity in male rats that was manifested by increased sperm abnormalities and deterioration of sperm motility, count and viability. NiO NPs also increased lipid peroxidation and negatively affected the cellular antioxidant defense system in the testis of rats. The level of serum testosterone hormone was increased in NiO NPs-exposed rats. qPCR showed a marked downregulation in expression of steroidogenesis-related genes (CYP11A1, HSD3B and STAR) and a significant upregulation in expression of apoptosis-related gene (caspase-9) in testicular tissue of rats. Various pathological lesions and an increase in the number of PCNA-positive immune-reactive cells were also noticed in the testis of NiO NPs-exposed rats. Co-administration of HSP significantly ameliorated most of the NiO NPs-induced testicular damages and improved male fertility in rats.
随着氧化镍纳米粒子（NiO NPs）制造和应用的最新进展，人们对其不良影响的担忧也在增加。橙皮苷（HSP）是一种柑橘类黄酮，具有很强的抗炎、抗氧化和自由基清除活性。本研究旨在探讨橙皮苷对雄性大鼠睾丸和精子发生损伤的保护作用。40 只大鼠随机平均分为四组：对照组、NiO NPs 组、HSP 组和 NiO NPs+HSP 组。NiO NPs（100mg/kg）和/或 HSP（100mg/kg）通过灌胃每天给予 60 天。暴露于 NiO NPs 导致雄性大鼠产生明显的生殖毒性，表现为精子畸形增加和精子运动、计数和活力恶化。NiO NPs 还增加了睾丸组织中的脂质过氧化作用，并对细胞抗氧化防御系统产生负面影响。暴露于 NiO NPs 的大鼠血清睾酮激素水平升高。qPCR 显示，大鼠睾丸组织中与类固醇生成相关的基因（CYP11A1、HSD3B 和 STAR）的表达明显下调，与凋亡相关的基因（caspase-9）的表达显著上调。在 NiO NPs 暴露的大鼠睾丸中还观察到各种病理损伤和 PCNA 阳性免疫反应细胞数量增加。HSP 的共同给药显著改善了 NiO NPs 引起的大多数睾丸损伤，并提高了大鼠的雄性生育能力。
