PG & Research Department of Chemistry, C. Abdul Hakeem College (Autonomous), Affiliated to Thiruvalluvar University, Melvisharam, Tamil Nadu, India.
Aquatic Animal Health Laboratory, PG & Research Department of Zoology, C. Abdul Hakeem College (Autonomous), Affiliated to Thiruvalluvar University, Melvisharam, Tamil Nadu, India.
Chemosphere. 2022 Sep;303(Pt 1):134891. doi: 10.1016/j.chemosphere.2022.134891. Epub 2022 May 12.
Carbon nanotubes (CNTs) have been widely used in developing polymer hybrid coatings for anticorrosive application. In the present study, poly [(3,5-dimethyl-lH-pyrazole-1-yl) methyl methacrylate-co-glycidyl methacrylate] (PyM) was prepared by solution polymerization. Single-wall carbon nanotubes (SWCNT) were incorporated in the PyM by solution blending technique at different proportions. The PyM and its SWCNT (PyM-SWCNT) nanocomposites were characterized by FT-IR spectroscopy, X-Ray Diffraction, FE-SEM and HR-TEM. Different concentrations of PyM or PyM-SWCNT prepared in the present study were assessed separately for their toxicity by in vivo and in vitro assays using zebrafish embryos and gill cell line of zebrafish (DrG), respectively. The nanocomposites at the concentration of 400 μg ml of PyM in 1.0% of SWCNT was found to be non-toxic and recommended for anticorrosive application whereas the nanocomposites with above 1% of SWCNT was found to be toxic. The nanocomposites with 1.5% of SWCNT delayed the hatching rate of eggs, decreased survival rate and heart beat in zebrafish embryos, and induced the morphological changes in DrG cells. Gene expression studies revealed that PyM-SWCNT with high concentration of SWCNT induced oxidative stress by activating ROS generations in zebrafish embryos and DrG cells. The immersion study of uncoated and coated with recommended concentration of PyM-SWCNT on mild steel (MS) in sea water was studied using FE-SEM and EDS, and the results showed effective corrosion protection without leaching behaviour. The nanocomposites with novel polymer in the present study may be used in the industry for anticorrosive purpose.
碳纳米管 (CNTs) 已广泛应用于开发聚合物杂化涂层以实现防腐应用。在本研究中，通过溶液聚合制备了聚[(3,5-二甲基-1H-吡唑-1-基)甲基甲基丙烯酸酯-co-甲基丙烯酸缩水甘油酯]（PyM）。通过溶液共混技术将单壁碳纳米管 (SWCNT) 以不同比例掺入 PyM 中。通过傅里叶变换红外光谱、X 射线衍射、FE-SEM 和高分辨率透射电子显微镜对 PyM 及其 SWCNT（PyM-SWCNT）纳米复合材料进行了表征。使用斑马鱼胚胎和斑马鱼鳃细胞系（DrG）分别通过体内和体外测定评估了本研究中制备的不同浓度的 PyM 或 PyM-SWCNT 的毒性。发现浓度为 400μg/ml 的 PyM 在 1.0%的 SWCNT 纳米复合材料是无毒的，推荐用于防腐应用，而高于 1%的 SWCNT 的纳米复合材料是有毒的。含有 1.5%SWCNT 的纳米复合材料延迟了卵的孵化率，降低了斑马鱼胚胎的存活率和心跳，并诱导 DrG 细胞发生形态变化。基因表达研究表明，高浓度 SWCNT 的 PyM-SWCNT 通过在斑马鱼胚胎和 DrG 细胞中激活 ROS 的产生而诱导氧化应激。在海水中对未涂层和涂覆有推荐浓度的 PyM-SWCNT 的低碳钢 (MS) 进行了浸泡研究，使用 FE-SEM 和 EDS 进行了研究，结果表明在没有浸出行为的情况下具有有效的腐蚀保护。本研究中具有新型聚合物的纳米复合材料可用于工业中的防腐目的。
