Department of Neurology, First Affiliated Hospital, Kunming Medical University, 295 Xi Chang Road, Kunming, Yunnan 650032, PR China.
Department of Neurology, Northeast Yunnan Hospital, Mengquan Avenue, Zhaoyang District, Zhaotong, Yunnan 657000, PR China.
Neuroscience. 2022 Jul 1;494:69-81. doi: 10.1016/j.neuroscience.2022.05.009. Epub 2022 May 13.
Augmentation of neurogenesis and migration of newly born neurons into ectopic regions like the hilus play critical roles during the pathophysiology of acute kindled seizures. Evidence shows that disrupted in schizophrenia 1 (DISC1) has an influence on adult neurogenesis in the dentate gyrus (DG); however, its role of regulating neurogenesis and mispositioned newborn neurons in the hilus after status epilepticus (SE) remains unknown. Using double immunofluorescence staining, the present study clarifies that DISC1 is co-expressed with nearly all of the neuronal markers, which are characterized by different stages of neuronal development, after pilocarpine-induced SE in mice. This reveals that DISC1 takes part in the modulation of neurogenesis in the hilus post-SE. Unexpectedly, an interesting phenomenon was observed as well. Some glial fibrillary acidic protein (GFAP)-positive cells in the hilus appeared to encircle the DISC1-positive cells, which possibly indicated that DISC1 may participate in the process of neuronal or neural development associated with astrocytes such as phagocytosis, dendritic spine development, synaptic transmission, and developmental and synaptic plasticity.
神经发生的增强和新生神经元向海马伞等异位区域的迁移在急性点燃性癫痫发作的病理生理学中起着关键作用。有证据表明，分裂症相关蛋白 1（DISC1）会影响齿状回（DG）中的成年神经发生；然而，其在癫痫持续状态（SE）后调节神经发生和新生神经元异位的作用尚不清楚。本研究通过双重免疫荧光染色，明确了在匹罗卡品诱导的 SE 后，DISC1 与几乎所有神经元标志物在小鼠海马伞中共同表达，这些标志物具有不同的神经元发育阶段。这表明 DISC1 参与了 SE 后海马伞中神经发生的调节。出乎意料的是，还观察到了一个有趣的现象。海马伞中一些胶质纤维酸性蛋白（GFAP）阳性细胞似乎包围着 DISC1 阳性细胞，这可能表明 DISC1 可能参与了与星形胶质细胞相关的神经元或神经发育过程，如吞噬作用、树突棘发育、突触传递以及发育和突触可塑性。
