Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul 08826, Republic of Korea.
Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea.
Int J Biol Macromol. 2022 Jun 30;211:535-544. doi: 10.1016/j.ijbiomac.2022.05.074. Epub 2022 May 13.
The root bark of Ulmus davidiana var. japonica (Rehder) Nakai (Japanese elm) has been used for inflammatory disease treatments. In this work, we isolated pectic polysaccharides from the root bark of U. davidiana (UDP) and explored the immune activities of intact and ultrasonicated UDP on human macrophages. The UDP-treated macrophages showed a proinflammatory response, indicating classical activation via Toll-like receptor-mediated recognition. For hydrogel formation, the ultrasonicated UDP was modified with methacrylate groups, then subjected to photocrosslinking. The formed bulk hydrogel was pulverized into microgels by homogenization, and the microgel size was modulated for macrophage phagocytosis. The UDP microgel-treated macrophages displayed microgel internalization and classical activation that involved upregulation of M1 polarization markers (IL6, TNF-α, and CCR7), indicating that the microgel can be used as a carrier for macrophage-targeted drug delivery.
日本榆（Ulmus davidiana var. japonica (Rehder) Nakai）的根皮被用于治疗炎症性疾病。在这项工作中，我们从日本榆的根皮中分离出果胶多糖（UDP），并探索了完整的和超声处理的 UDP 对人巨噬细胞的免疫活性。UDP 处理的巨噬细胞表现出促炎反应，表明通过 Toll 样受体介导的识别发生经典激活。为了形成水凝胶，将超声处理的 UDP 用甲基丙烯酰基修饰，然后进行光交联。形成的块状水凝胶通过匀浆粉碎成微凝胶，并调节微凝胶的大小以用于巨噬细胞吞噬。UDP 微凝胶处理的巨噬细胞显示出微凝胶内化和经典激活，涉及 M1 极化标志物（IL6、TNF-α 和 CCR7）的上调，表明微凝胶可用作巨噬细胞靶向药物递送的载体。
