Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Chair of Urban Water Systems Engineering, Technical University of Munich, Am Coulombwall 3, 85748 Garching, Germany.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China; Water Chemistry and Water Technology, Engler-Bunte-Institut, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Bioresour Technol. 2022 Jul;356:127310. doi: 10.1016/j.biortech.2022.127310. Epub 2022 May 13.
Two-stage Partial nitritation/Anammox (PN/A) was firstly performed for recalcitrant organics (RO)-rich thermal hydrolysis pretreated anaerobic digestion (THP-AD) centrate treatment with municipal wastewater (MW) as co-substrate. Results indicated the inhibitory effects of RO was alleviated and high nitrate issue in PN/A effluent was addressed by cotreatment strategy. Stable PN with nitrite accumulation ratio of 95% and N removal efficiency of 97.1% were well maintained at MW of 80%. Nevertheless, nitrate accumulation and anammox activity loss were observed with lowering MW proportion owing to the weakened denitrification activity and aggravated inhibitory effect. Microbial analysis revealed Nitrosomonas was the major ammonium oxidizing bacteria and the ideal PN performance was due to the effective out-selection of nitrite oxidizing bacteria. Candidatus Kuenenia was identified as the primary bacteria for nitrogen removal (82.7%), and the controlled abundance of heterotrophic denitrifiers in anammox system ensured the enhanced nitrogen removal regardless of high COD loading from THP-AD centrate.
两段式部分亚硝化/厌氧氨氧化（PN/A）首先用于处理含有难降解有机物（RO）的热水解预处理厌氧消化（THP-AD）浓缩液，市政污水（MW）作为共基质。结果表明，共处理策略缓解了 RO 的抑制作用，并解决了 PN/A 出水中的高硝酸盐问题。在 MW 为 80%的条件下，稳定的 PN 保持了 95%的亚硝酸盐积累比和 97.1%的氮去除效率。然而，随着 MW 比例的降低，观察到硝酸盐积累和厌氧氨氧化活性损失，这是由于反硝化活性减弱和抑制作用加剧所致。微生物分析表明，亚硝化单胞菌是主要的氨氧化菌，理想的 PN 性能是由于有效选择了亚硝酸盐氧化菌。卡氏菌被鉴定为主要的脱氮菌（82.7%），并且在厌氧氨氧化系统中控制异养反硝化菌的丰度，确保了即使来自 THP-AD 浓缩液的 COD 负荷较高，也能增强脱氮效果。
