Institute of Eco-Environmental Forensics, Qingdao Institute of Humanities and Social Sciences, Shandong University, China.
Institute of Eco-Environmental Forensics, Qingdao Institute of Humanities and Social Sciences, Shandong University, China.
Anal Chim Acta. 2022 May 29;1209:339893. doi: 10.1016/j.aca.2022.339893. Epub 2022 May 2.
With their advantages in specificity, high stability and easy screening, aptamers are becoming increasingly popular recognition elements for biosensor platforms. At the same time, microchips as the new analytical detection platforms have achieved significant growth in the past decades. At present, with the intersection of aptamer and microfluidic technology, aptamer-based high-sensitivity bioanalysis on microchips exhibits a great application potential in biomedical science and environmental fields. In this review, we highlight the recent progress in high-sensitivity bioanalytical applications based on aptamer signal amplification strategies on microchips. Furthermore, the main challenges in the practical application are discussed, and the development in the future is prospected.
凭借其特异性、高稳定性和易于筛选的优势，适体正成为生物传感器平台越来越受欢迎的识别元件。与此同时，微芯片作为新的分析检测平台，在过去几十年中取得了显著的发展。目前，随着适体与微流控技术的交叉，基于适体的微芯片上高灵敏度生物分析在生物医学和环境领域展现出巨大的应用潜力。在本文中，我们重点介绍了基于适体信号放大策略在微芯片上进行高灵敏度生物分析的最新进展。此外，还讨论了实际应用中的主要挑战，并对未来的发展进行了展望。
