Xue Jing, Chen Feng, Bai Min, Cao Xiaowen, Fu Wenhao, Zhang Jin, Zhao Yongxi
Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xianning West Road, Xi'an, Shaanxi 710049, P. R. China.
ACS Appl Mater Interfaces. 2021 Mar 3;13(8):9402-9411. doi: 10.1021/acsami.0c16138. Epub 2020 Nov 10.
Aptamers have drawn great attention in the field of biological research and disease diagnosis for the remarkable advantages as recognition elements. They show unique superiority for facile selection, desirable thermal stability, flexible engineering, and low immunogenicity, complementing the use of conventional antibodies. Aptamer-functionalized microdevices offer promising properties for bioanalysis applications because of the compact sizes, minimal reaction volume, high throughput, operational feasibility, and controlled preciseness. In this review, we first introduce the innovative technologies in the selection of aptamers with microdevices and then highlight some advanced applications of aptamer-functionalized microdevices in bioanalysis field for diverse targets. Aptamer-functionalized microfluidic devices, microarrays, and paper-based and other interface-based microdevices are all bioanalysis platforms with huge potential in the near future. Finally, the major challenges of these microdevices applied in bioanalysis are discussed and future perspectives are also envisioned.
适配体作为识别元件具有显著优势，在生物研究和疾病诊断领域引起了极大关注。它们在便捷筛选、良好的热稳定性、灵活的工程改造和低免疫原性方面展现出独特优势，可作为传统抗体的补充。适配体功能化的微器件由于尺寸紧凑、反应体积小、高通量、操作可行性高和控制精度高，为生物分析应用提供了有前景的特性。在这篇综述中，我们首先介绍了利用微器件筛选适配体的创新技术，然后重点介绍了适配体功能化微器件在生物分析领域针对不同靶标的一些先进应用。适配体功能化的微流控器件、微阵列以及基于纸和其他界面的微器件都是在不久的将来具有巨大潜力的生物分析平台。最后，讨论了这些微器件应用于生物分析的主要挑战，并展望了未来前景。
