Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo 542/2, 166 10, Prague 6, Czech Republic.
Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo 542/2, 166 10, Prague 6, Czech Republic.
Anal Chim Acta. 2022 May 29;1209:339447. doi: 10.1016/j.aca.2022.339447. Epub 2022 Jan 7.
This paper provides a detailed overview of relevant developments in separation and analysis of proteins by capillary electromigration (CE) methods in the period 2017-mid 2021. The presented topics embrace sample preparation, suppression of protein sorption, control of electroosmotic flow, separations of proteins by the particular CE methods and protein identification and quantification by various detection techniques. To illustrate the wide usability of CE methods for protein analysis, the paper shows several novel applications of CE methods, such as quality control and characterization of protein biopharmaceuticals, determination of proteins in complex biomatrices, investigation of posttranslational modification, peptide mapping, interactions of proteins with other (bio)molecules and determination of their physicochemical and biochemical characteristics.
本文综述了 2017 年至 2021 年中期期间，基于毛细管电泳（CE）方法分离和分析蛋白质的相关进展。呈现的主题包括样品制备、抑制蛋白质吸附、控制电动流、通过特定 CE 方法分离蛋白质以及通过各种检测技术进行蛋白质鉴定和定量。为了说明 CE 方法在蛋白质分析中的广泛适用性，本文展示了 CE 方法的几个新应用，例如蛋白质生物药物的质量控制和表征、复杂生物基质中蛋白质的测定、翻译后修饰的研究、肽图分析、蛋白质与其他（生物）分子的相互作用及其物理化学和生化特性的测定。
