Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
Department of Pharmaceutical Technology, Adamas University, Barasat, Kolkata, West Bengal, India.
Curr Drug Saf. 2023;18(2):214-223. doi: 10.2174/1574886317666220514153858.
Drymaria cordata (Linn.) Willd is a creeping herb belonging to the Caryophyllaceae family, widely used as a traditional medicine in Africa and North-east India for various ailments. Many therapeutic applications of D. cordata have been reported in various scientific studies, but the teratogenicity study of this herb has not been documented till now.
The present study aimed to assess the developmental toxic effect and median lethal concentration (LC) of methanol extract of Drymaria cordata leaf (DCME) using zebrafish embryos. After spawning of male and female zebrafish, healthy zebrafish embryos were selected by microscopic screening and transferred into 96-well plate for the study. Embryos were exposed to DCME at concentrations ranging from 50-400 μg/ml in 2% DMSO from 24 hpf to 72 hpf.
Developmental and morphological abnormalities were microscopically evaluated. Fifty percent lethal concentration (LC) of DCME was determined by observation from 24 hpf to 72 hpf. The concentration-dependent toxic effects of DCME on developing embryos of zebrafish were found in the study in a time-dependent manner.
At 72 hpf, the median lethal concentration (LC) of DCME was found with visible developmental defects, such as heartbeat rate, less pigmentation, oedema, spinal curvature, immature yolk sac as well as reduced hatching rate and a slow growth. The median lethal dose was found to be 448 μg/ml at 72 hpf for zebra fish embryos, meriting further studies on toxicological profiling of the plants.
卷柏（Drymaria cordata（Linn.）Willd）是石竹科的一种匍匐草本植物，在非洲和印度东北部被广泛用作传统药物，用于治疗各种疾病。在各种科学研究中已经报道了许多关于卷柏的治疗应用，但到目前为止，还没有关于该草药致畸性的研究。
本研究旨在评估甲醇提取物对斑马鱼胚胎的发育毒性作用和半数致死浓度（LC）。雌雄斑马鱼产卵后，通过显微镜筛选选择健康的斑马鱼胚胎，并将其转移到 96 孔板中进行研究。胚胎在 24 hpf 至 72 hpf 期间，用浓度范围为 50-400 μg/ml 的 2%二甲基亚砜（DMSO）处理 DCME。
通过显微镜评估了发育和形态异常。通过观察从 24 hpf 到 72 hpf 确定了 DCME 的 50%致死浓度（LC）。研究发现，DCME 对斑马鱼胚胎的发育毒性作用呈浓度依赖性，且具有时间依赖性。
在 72 hpf 时，观察到可见的发育缺陷，如心跳率、色素沉着减少、水肿、脊柱弯曲、未成熟卵黄囊以及孵化率降低和生长缓慢，此时 DCME 的半数致死浓度（LC）为 448 μg/ml。斑马鱼胚胎，值得进一步研究该植物的毒理学特征。
