Medical School, Zhejiang University City College, Hangzhou, China.
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
Phytochem Anal. 2022 Aug;33(6):851-868. doi: 10.1002/pca.3134. Epub 2022 May 16.
Actinidia eriantha Benth radix (AEBR) is one of the most commonly used medicines by the She people in China, used primarily for the treatment of tumours of the digestive tract. There is currently limited to no data on the quality control of AEBR.
The aim of this study was to identify quality markers of AEBR.
An ultra-performance lquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) method was used to identify and analyse the components of AEBR from water extracts. An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was also established for the simultaneous determination of 13 active components in the water extracts. The network pharmacology method was used to screen for quality markers of AEBR in gastric cancer.
This study tentatively identified 199 chemical constituents and isomers, including 67 pentacyclic triterpenoids, 20 flavonoids, 39 phenolic acids, 18 coumarins, and other compounds. The 13 active components in the water extracts were successfully determined using a validated UPLC-MS/MS method. Based on the network pharmacology method, four compounds were selected as quality markers of AEBR.
This study provides an important reference for the quality control of AEBR. Chemical analysis combined with network pharmacology provides an effective strategy for the discovery of quality markers in traditional Chinese/herb medicine.
藤梨根（AEBR）是中国畲族常用药材之一，主要用于治疗消化道肿瘤。目前，AEBR 的质量控制数据有限。
本研究旨在鉴定 AEBR 的质量标志物。
采用超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF-MS）法对水提物中的 AEBR 成分进行鉴定和分析。还建立了超高效液相色谱-串联质谱（UPLC-MS/MS）法同时测定水提物中 13 种活性成分。采用网络药理学方法筛选 AEBR 治疗胃癌的质量标志物。
本研究初步鉴定了 199 种化学成分和异构体，包括 67 种五环三萜、20 种黄酮类、39 种酚酸、18 种香豆素和其他化合物。采用验证的 UPLC-MS/MS 方法成功测定了水提物中的 13 种活性成分。基于网络药理学方法，选择了四种化合物作为 AEBR 的质量标志物。
本研究为 AEBR 的质量控制提供了重要参考。化学分析结合网络药理学为中药/草药质量标志物的发现提供了有效的策略。
