Pandunugrahadi Muhammad, Irianto Komang Agung, Sindrawati Oen
Orthopaedic and Traumatology Department, Dr Soetomo General Hospital/Faculty of Medicine, Airlangga University, Surabaya, Indonesia.
Pathologic Anatomy Department, Faculty of Medicine, Widya Mandala Catholic University, Surabaya, Indonesia.
Int J Biomater. 2022 May 6;2022:9601547. doi: 10.1155/2022/9601547. eCollection 2022.
Without appropriate treatment, nerve injuries may result in permanent loss of function. Platelet-rich plasma (PRP) injection is found to help in nerve regeneration. PRP is a concentrated platelet derived from autologous blood with the potential to release various growth factors (GF) to promote nerve regeneration. This study aims to know the best time for PRP injection to promote nerve regeneration.
This is an experimental in vivo research using male New Zealand white rabbits in the randomized control group posttest only design. Samples were divided into 5 groups (1 control group and 4 treatment groups). The control group without PRP injection and treated groups injected immediately after nerve injury, 3 days, 7 days, and 14 days afterward. Nerve regeneration was evaluated by the histology specimen sacrificed on day 21. Inflammation cells and endoneurium vacuoles were counted as mean percentage of five nerve fragments in each injured nerve sample specimen.
Inflammation cells and vacuole cells increased significantly when PRP was administered 3 days after injury (group 2) (respectively, 14 ± 6.7 and 56.6 ± 11.6) compared to all treatment groups ( < 0.005) (control group, respectively, 6 ± 2.6 and 15.7 ± 9.5). On the other hand, significantly lower endoneurium vacuoles and inflammation cells were found on "the day 14" sample group (respectively, 5 ± 1.3 and 5.2 ± 1.6) compared to all other groups ( < 0.005).
This study found that the best time for injecting PRP for nerve regeneration is 14 days after injury.
若未经适当治疗，神经损伤可能导致功能永久性丧失。研究发现，注射富血小板血浆（PRP）有助于神经再生。PRP是一种从自体血液中提取的浓缩血小板，具有释放多种生长因子（GF）以促进神经再生的潜力。本研究旨在确定PRP注射促进神经再生的最佳时机。
这是一项体内实验研究，采用雄性新西兰白兔，仅设随机对照组的后测设计。样本分为5组（1个对照组和4个治疗组）。对照组不注射PRP，治疗组分别在神经损伤后即刻、3天、7天和14天注射。于第21天处死动物获取组织学标本，评估神经再生情况。对每个损伤神经样本标本中的五个神经片段，计数炎症细胞和神经内膜空泡，并计算其平均百分比。
与所有治疗组相比，损伤后3天注射PRP的组（第2组）炎症细胞和空泡细胞显著增加（分别为14±6.7和56.6±11.6）（P<0.005）（对照组分别为6±2.6和15.7±9.5）。另一方面，与所有其他组相比，“第14天”样本组的神经内膜空泡和炎症细胞显著减少（分别为5±1.3和5.2±1.6）（P<0.005）。
本研究发现，注射PRP促进神经再生的最佳时机是损伤后14天。
