使用单分子实时测序技术评估α-和β-发酵的内生真菌组成。
Assessment of the Endophytic Fungal Composition of and -Fermented Using Single-Molecule, Real-Time Sequencing Technology.
作者信息
Zhang Liheng, Li Xianghui, Song Xinghui, Bian Chuanzhou, Kang Xiangtao, Zhao Junqiang, Qiao Hongxing, Gong Yanzhang
机构信息
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, China.
出版信息
Front Vet Sci. 2022 Apr 28;9:880152. doi: 10.3389/fvets.2022.880152. eCollection 2022.
Endophytic fungus represents microorganisms existing within the healthy plant organs, which can significantly influence metabolic product production in plants, a process with great research value and broad prospects for development. To investigate the effect of fermentation with probiotic cultures on the endophytic fungal diversity and composition of , we used single-molecular, real-time sequencing (Pacific Biosciences) for 18S ribosomal RNA (rRNA) sequencing. The results showed that the endophytic fungi of mainly belonged to , and . Furthermore, the endophytic fungal diversity and abundance of were more variable after fermentation with . Our data lays a solid and comprehensive foundation for further exploration of endophytic fungi from as potential sources of functional compounds.
内生真菌是指存在于健康植物器官内的微生物，其可显著影响植物代谢产物的产生，这一过程具有很高的研究价值和广阔的发展前景。为了研究益生菌发酵对[植物名称]内生真菌多样性和组成的影响，我们使用单分子实时测序（PacBio）对18S核糖体RNA（rRNA）进行测序。结果表明，[植物名称]的内生真菌主要属于[具体真菌类别1]、[具体真菌类别2]和[具体真菌类别3]。此外，[植物名称]经[发酵剂名称]发酵后，内生真菌的多样性和丰度变化更大。我们的数据为进一步探索[植物名称]内生真菌作为功能性化合物潜在来源奠定了坚实而全面的基础。
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