Dong Tina T X, Ma Xiao Q, Clarke Clarles, Song Zong H, Ji Zhao N, Lo Chun K, Tsim Karl W K
Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China.
J Agric Food Chem. 2003 Nov 5;51(23):6709-14. doi: 10.1021/jf034278x.
Radix Astragali (root of Astragalus; Huangqi) is a traditional Chinese medicine commonly used as an immunostimulant, hepatoprotective, diuretic, antidiabetic, analgesic, expectorant, and sedative drug. Although the species of Radix Astragali have been defined as Astragalus membranaceus and A. membranaceus var. mongholicus in Pharmacopoeia of China, their taxonomy remains controversial. The phylogenetic relationships among 10 Astragalus taxa, which are commonly found in China including A. membranaceus, A. membranaceus var. mongholicus, Astragalus propinquus, Astragalus lepsensis, Astragalus aksuensis, Astragalus hoantchy, Astragalus hoantchy subsp. dshimensis,Astragalus lehmannianus, Astragalus sieversianus, and Astragalus austrosibiricus, were determined using the DNA sequences of the 5S ribosomal RNA (5S rRNA) spacer, internal transcribed spacer region (ITS), and 18S rRNA coding region. The 5S rRNA spacer, ITS, and 18S rRNA, amplified by polymerase chain reaction from the isolated genomic DNAs, were sequenced. By using neighbor-joining and maximum parsimony analyses, phylogenetic trees were mapped by their sequence diversity. A. membranaceus and A. membranaceus var. mongholicus shared the greatest sequence homology. In addition, A. propinquus shared a closer relationship with A. membranaceus and A. membranaceus var. mongholicus, while other Astragalus species were less closely related. This is the first paper to show the phylogenetic relationship of Astragalus species related to Radix Astragali in China by the molecular genetic approach.
黄芪是一种传统中药，常用作免疫刺激剂、肝脏保护剂、利尿剂、抗糖尿病药、镇痛药、祛痰药和镇静药。虽然在中国药典中黄芪的物种已被定义为蒙古黄芪和膜荚黄芪，但它们的分类学仍存在争议。利用5S核糖体RNA（5S rRNA）间隔区、内转录间隔区（ITS）和18S rRNA编码区的DNA序列，确定了在中国常见的10个黄芪类群之间的系统发育关系，这些类群包括膜荚黄芪、蒙古黄芪、库伦黄芪、长荚黄芪、阿克苏黄芪、贺兰山黄芪、贺兰山黄芪石敏亚种、莱氏黄芪、西氏黄芪和西伯利亚黄芪。通过聚合酶链反应从分离的基因组DNA中扩增出5S rRNA间隔区、ITS和18S rRNA，并进行测序。通过邻接法和最大简约法分析，根据它们的序列多样性绘制了系统发育树。膜荚黄芪和蒙古黄芪具有最大的序列同源性。此外，库伦黄芪与膜荚黄芪和蒙古黄芪的关系更为密切，而其他黄芪物种的关系则较远。这是第一篇通过分子遗传学方法展示中国与黄芪相关的黄芪物种系统发育关系的论文。
