Uzair Muhammad, Ali Mohsin, Fiaz Sajid, Attia Kotb, Khan Naeem, Al-Doss Abdullah A, Ramzan Khan Muhammad, Ali Zulfiqar
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.
National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad 45500, Pakistan.
Saudi J Biol Sci. 2022 Jun;29(6):103299. doi: 10.1016/j.sjbs.2022.103299. Epub 2022 Apr 26.
Salinity affects plant growth, development, yield, and is a big challenge for wheat growth across the globe. Possible feasible solution is creation of salt-tolerant material, genetic variation is a criterion to developing genetically superior individuals. To assess the genetic variation for salt tolerance, nationally and internationally-derived 81 wheat genotypes were selected and evaluated in 0- and 150-mM salt in nutritional culture at seedling stage. Results indicate that salinity levels reveal significant ( ≤ 0.01) differences for fresh root weight (RW), shoot length (SL), fresh shoot weight (SW), total plant length (TL), total fresh weight (TW), root/shoot weight ratio (RSWR), root/shoot length ratio (RSLR), and relative growth rate for weight (RGR-Wt). While, there was no difference for root length (RL). Hierarchical Clustering and Pairwise correlation analysis showed TW, RGR-Wt, SL, SW, and RW were positively correlated among themselves, whereas RL had poor correlations with all the traits except TL and RSLR. Hence, selection of SL can improve the performance of other parameters. Based on PCA analysis, SW and RGR-Wt were the major discriminative components for wheat genotypes. Present study explained that shoot related parameters could be used as a selection criterion to categorize salt-tolerant genotypes. Outperforming genotypes 1104 and 1106 in saline conditions could be used as parents in creation of salt-tolerant wheat genotypes, and parameters such as SL, SW, TW, and RGR-Wt for early screening will be important for creating salt-tolerant and high yielding wheat genotypes.
盐度会影响植物的生长、发育和产量,是全球小麦生长面临的一大挑战。可能可行的解决办法是培育耐盐材料,遗传变异是培育遗传上更优良个体的一个标准。为了评估耐盐性的遗传变异,选取了来自国内和国际的81个小麦基因型,并在幼苗期于营养液中0 mM和150 mM盐浓度条件下进行评估。结果表明,盐度水平在鲜根重(RW)、地上部长度(SL)、鲜地上部重量(SW)、植株总长度(TL)、总鲜重(TW)、根/地上部重量比(RSWR)、根/地上部长度比(RSLR)以及重量相对生长率(RGR-Wt)方面显示出显著(≤0.01)差异。而根长(RL)没有差异。层次聚类和成对相关性分析表明,TW、RGR-Wt、SL、SW和RW之间呈正相关,而RL与除TL和RSLR之外的所有性状相关性较差。因此,选择SL可以改善其他参数的表现。基于主成分分析,SW和RGR-Wt是区分小麦基因型的主要成分。本研究表明,地上部相关参数可作为对耐盐基因型进行分类的选择标准。在盐胁迫条件下表现优异的基因型1104和1106可作为培育耐盐小麦基因型的亲本,而SL、SW、TW和RGR-Wt等参数对于早期筛选以培育耐盐高产小麦基因型至关重要。