Wang Yakun, Wei Jie, Hong Kunhao, Zhou Nan, Liu Xiaoli, Hong Xiaoyou, Li Wei, Zhao Jian, Chen Chen, Wu Liang, Yu Lingyun, Zhu Xinping
Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Front Physiol. 2022 Apr 29;13:885035. doi: 10.3389/fphys.2022.885035. eCollection 2022.
Salinity is a crucial factor influencing the growth, development, immunity, and reproduction of aquatic organisms; however, little is known about the molecular mechanism of the response to salinity challenge in larvae of the giant freshwater prawn . Herein, larvae cultured in three treatment groups with salinities of 10, 13, and 16‰ (S10, S13, and S16) were collected, and then transcriptome analysis was conducted by RNA-seq. A total of 6,473, 3,830 and 3,584 differentially expressed genes (DEGs) were identified in the S10 vs. S13 comparison, S10 vs. S16 comparison and S13 vs. S16 comparison, respectively. These genes are involved in osmoregulation, energy metabolism, molting, and the immune response. qPCR analysis was used to detect the expression patterns of 16 DEGs to verify the accuracy of the transcriptome data. Protein-protein interaction (PPI) analysis for DEGs and microsatellite marker screening were also conducted to reveal the molecular mechanism of salinity regulation. Together, our results will provide insight into the molecular genetic basis of adaptation to salinity challenge for larvae of .
盐度是影响水生生物生长、发育、免疫和繁殖的关键因素；然而，对于淡水大虾幼体应对盐度挑战的分子机制知之甚少。在此，收集了在盐度分别为10‰、13‰和16‰（S10、S13和S16）的三个处理组中培养的幼体，然后通过RNA测序进行转录组分析。在S10与S13比较、S10与S16比较以及S13与S16比较中，分别鉴定出6473个、3830个和3584个差异表达基因（DEG）。这些基因参与渗透调节、能量代谢、蜕皮和免疫反应。使用qPCR分析来检测16个DEG的表达模式，以验证转录组数据的准确性。还进行了DEG的蛋白质-蛋白质相互作用（PPI）分析和微卫星标记筛选，以揭示盐度调节的分子机制。总之，我们的结果将为深入了解淡水大虾幼体适应盐度挑战的分子遗传基础提供依据。
