Malik Adeel, Kim Chang-Bae
Institute of Intelligence Informatics Technology, Sangmyung University, Seoul 30316, Korea.
Department of Biotechnology, Sangmyung University, Seoul 30316, Korea.
Biology (Basel). 2021 Jan 8;10(1):39. doi: 10.3390/biology10010039.
Chinese mitten crab (CMC) or is a strong osmoregulator that can keep rigorous cellular homeostasis. CMC can flourish in freshwater, as well as seawater, habitats and represents the most important species for freshwater aquaculture. Salt stress can have direct effects on several stages (e.g., reproduction, molting, growth, etc.) of the CMC life cycle. To get a better overview of the genes involved in the gills of CMC under different salinity conditions, we conducted an RNA-Seq meta-analysis on the transcriptomes of four publicly available datasets. The meta-analysis identified 405 differentially expressed transcripts (DETs), of which 40% were classified into various transporter classes, including accessory factors and primary active transporters as the major transport classes. A network analysis of the DETs revealed that adaptation to salinity is a highly regulated mechanism in which different functional modules play essential roles. To the best of our knowledge, this study is the first to conduct a transcriptome meta-analysis of gills from crab RNA-Seq datasets under salinity. Additionally, this study is also the first to focus on the differential expression of diverse transporters and channels (transportome) in CMC. Our meta-analysis opens new avenues for a better understanding of the osmoregulation mechanism and the selection of potential transporters associated with salinity change.
中华绒螯蟹是一种强大的渗透调节者，能够维持严格的细胞内稳态。中华绒螯蟹既能在淡水环境中繁衍，也能在海水环境中生存，是淡水养殖中最重要的物种。盐胁迫会对中华绒螯蟹生命周期的多个阶段（如繁殖、蜕皮、生长等）产生直接影响。为了更好地了解不同盐度条件下中华绒螯蟹鳃中涉及的基因，我们对四个公开可用数据集的转录组进行了RNA测序元分析。元分析确定了405个差异表达转录本（DETs），其中40%被归类为各种转运蛋白类别，包括辅助因子和主要主动转运蛋白作为主要转运类别。对差异表达转录本的网络分析表明，对盐度的适应是一种高度调控的机制，其中不同的功能模块发挥着重要作用。据我们所知，本研究是首次对盐度条件下蟹类RNA测序数据集的鳃进行转录组元分析。此外，本研究也是首次关注中华绒螯蟹中多种转运蛋白和通道（转运组）的差异表达。我们的元分析为更好地理解渗透调节机制以及与盐度变化相关的潜在转运蛋白的选择开辟了新途径。
