Jiang Jingjing, Su Shengyan, Lai Ting, Feng Wenrong, Li Feifan, Tian Can, Gao Yang, Munganga Brian Peelekelo, Tang Yongkai, Xu Pao
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
Front Physiol. 2022 Apr 27;13:793699. doi: 10.3389/fphys.2022.793699. eCollection 2022.
Given the difficulty in identifying individuals with different degrees of ovarian development, we developed a new device utilizing the hypothesis of mutual attraction behavior between male and female crabs with mature gonads by releasing the sexual pheromone so they could be examined. From a total of 40 female crabs, 10 were isolated within half an hour. Histological analysis showed that the ovaries of crabs in the isolated group were in stage IV, while those of the control groups were in stage III. In addition, progesterone (PROG) in experimental groups was significantly reduced compared with the control group ( < 0.05), but no significant difference was detected in estradiol (E2). In response to the different developmental stages, hemolymph biochemical indices and the determination of gonadal fatty acids profiles were explored. The results indicated only C18:4 showed a significant difference between these two groups. A transcriptome was generated to determine the genes involved in the mutual attraction process; differentially expressed genes (DEGs) were significantly related to gonadal development. Therefore, the device can be used to isolate Chinese mitten crabs with stage IV ovarian development.
鉴于识别具有不同卵巢发育程度的个体存在困难，我们基于成熟性腺的雌雄蟹之间相互吸引行为的假说，开发了一种通过释放性信息素来检测它们的新装置。在总共40只雌蟹中，有10只在半小时内被分离出来。组织学分析表明，隔离组蟹的卵巢处于IV期，而对照组的处于III期。此外，实验组的孕酮（PROG）与对照组相比显著降低（<0.05），但雌二醇（E2）未检测到显著差异。针对不同发育阶段，探索了血淋巴生化指标和性腺脂肪酸谱的测定。结果表明，只有C18:4在这两组之间存在显著差异。生成了一个转录组来确定参与相互吸引过程的基因；差异表达基因（DEG）与性腺发育显著相关。因此，该装置可用于分离卵巢发育处于IV期的中华绒螯蟹。
