Motafeghi Farzaneh, Mortazavi Parham, Salman Mahiny Amir Hossein, Abtahi Mohammad Mehdi, Shokrzadeh Mohammad
Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Ramsar International Branch, Mazandaran University of Medical Sciences, Ramsar, Iran.
Drug Chem Toxicol. 2023 Nov;46(4):617-624. doi: 10.1080/01480545.2022.2075377. Epub 2022 May 16.
Oxidative stress plays a prominent role in expanding toxicity and various diseases. This study investigated the potential protective effects of ginger () rhizome extract and NAC on docetaxel induced genotoxicity and oxidative stress. The antioxidant power of NAC and ginger extract on the genetic toxicity induced by docetaxel was assessed by micronucleus test. The ROS test with DCFH reagent was used to assess the reactive oxygen species. The thiobarbituric acid method was used to evaluate the amount of MDA produced by docetaxel. The amounts of phenol and flavonoids in the ginger extracts were also evaluated. The amount of phenol in the ginger extract was 0.886 mg of gallic acid per gram of dry extract. The amount of flavonoids were 0.242 mg/mL of quercetin per gram of dry extract. As shown by the micronucleus results, concentrations of 100 and 500 μM NAC and all concentrations of the ginger extract significantly reduced the number of micronuclei produced by docetaxel. On the other hand, the results of oxidative stress tests (ROS and LPO) showed that docetaxel in HGF cells increased the production of ROS and LPO, and the concentrations of ginger extract and NAC decreased oxidative stress in HGF cells in a dose-dependent manner. The results indicate that using these two antioxidants helps inhibit genetic toxicity and oxidative stress caused by docetaxel.
氧化应激在扩大毒性和引发各种疾病方面起着重要作用。本研究调查了生姜根茎提取物和N-乙酰半胱氨酸（NAC）对多西他赛诱导的遗传毒性和氧化应激的潜在保护作用。通过微核试验评估NAC和生姜提取物对多西他赛诱导的遗传毒性的抗氧化能力。使用DCFH试剂进行活性氧（ROS）测试以评估活性氧的水平。采用硫代巴比妥酸法评估多西他赛产生的丙二醛（MDA）量。还评估了生姜提取物中的酚类和黄酮类化合物含量。生姜提取物中的酚类含量为每克干提取物含0.886毫克没食子酸。黄酮类化合物含量为每克干提取物含0.242毫克/毫升槲皮素。微核试验结果表明，100和500μM的NAC浓度以及生姜提取物的所有浓度均显著降低了多西他赛产生的微核数量。另一方面，氧化应激试验（ROS和脂质过氧化，LPO）结果表明，多西他赛在人正常肝细胞（HGF）中增加了ROS和LPO的产生，而生姜提取物和NAC的浓度以剂量依赖的方式降低了HGF细胞中的氧化应激。结果表明，使用这两种抗氧化剂有助于抑制多西他赛引起的遗传毒性和氧化应激。
