Motafeghi Farzaneh, Mortazavi Parham, Shokrzadeh Mohammad
Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences and Metabolism, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Toxicol Res (Camb). 2024 Jan 16;13(1):tfad127. doi: 10.1093/toxres/tfad127. eCollection 2024 Feb.
Considering the numerous drug resistance in cancer and the advancement of science in nanomedicines, it was decided to compare the effectiveness of zinc oxide nanoparticles in colon and prostate cell lines. Considering the importance of factors and Oxidative stress pathways in cancer prevention, the aim of the study is based on oxidative stress mechanisms.
In order to evaluate the effects of zinc oxide nanoparticles on colon and prostate cell lines, oxidative stress factors ROS, MDA, and GSH and mitochondrial function were evaluated. The data was analyzed with Prism v8 software, and the significance level was considered to be < 0.05.
The results showed that nanoparticles induce ROS and reduce intracellular glutathione by destroying and disrupting mitochondrial function, and by increasing ROS production, damage to the lipid membrane and an increase in MDA were also evident. This effect was dose-dependent and the greatest at a concentration of 25 μg/mL. Also, ZnO nanoparticles performed better in the HT29 cell line than in the PC3 cell line.
This study showed that exposure of HT29 and PC3 cancer cells to zinc oxide nanoparticles at different concentrations inhibited growth by cytotoxic effects.
鉴于癌症中存在众多耐药性以及纳米医学领域的科学进展，决定比较氧化锌纳米颗粒在结肠和前列腺细胞系中的有效性。考虑到各种因素以及氧化应激途径在癌症预防中的重要性，本研究旨在基于氧化应激机制展开。
为评估氧化锌纳米颗粒对结肠和前列腺细胞系的影响，对氧化应激因子活性氧（ROS）、丙二醛（MDA）、谷胱甘肽（GSH）以及线粒体功能进行了评估。数据采用Prism v8软件进行分析，显著性水平设定为<0.05。
结果表明，纳米颗粒通过破坏和扰乱线粒体功能诱导ROS产生并降低细胞内谷胱甘肽水平，且随着ROS生成增加，脂质膜损伤以及MDA水平升高也很明显。这种效应呈剂量依赖性，在浓度为25μg/mL时最为显著。此外，氧化锌纳米颗粒在HT29细胞系中的表现优于PC3细胞系。
本研究表明，不同浓度的氧化锌纳米颗粒作用于HT29和PC3癌细胞时，通过细胞毒性作用抑制了细胞生长。
