Cardiovascular Physiology Laboratory, Universidade Federal do Pampa, BR 472, Km 592, Uruguaiana, Rio Grande do Sul, Brazil.
Department of Basic Health Sciences, Universidad Rey Juan Carlos, Atenas s/n, Alcorcón, Spain.
Food Funct. 2022 Jun 6;13(11):5996-6007. doi: 10.1039/d2fo00561a.
: To investigate the effects of egg white hydrolysate (EWH) on the lipid and glycemic metabolism disruption in the white adipose tissue (WAT) dysfunction induced by mercury (Hg). : Wistar rats were treated for 60 days: control (saline, intramuscular - i.m.); hydrolysate (EWH, gavage, 1 g kg day); mercury (HgCl, i.m., 1 dose 4.6 μg kg, subsequent doses 0.07 μg kg day) and hydrolysate-mercury (EWH-HgCl). Hg level and histological analyses were performed in epididymal WAT (eWAT), pancreas and liver. GRP78, CHOP, PPARα, PPARγ, leptin, adiponectin, and CD11 mRNA expressions were analyzed in eWAT. The plasma lipid profile, glucose, and insulin levels were measured. Antioxidant status was also evaluated in the plasma and liver. : EWH intake prevented the reduced eWAT weight, adipocyte size, insulin levels, and antioxidant defenses and the increased glucose and triglyceride levels induced by Hg exposure; hepatic glutathione levels were higher in rats co-treated with EWH. The increased mRNA expression of CHOP, PPARα, and leptin induced by Hg was reduced in co-treated rats. EWH did not modify the elevated mRNA expression of GRP78, PPARγ and adiponectin in Hg-treated rats. Increased levels of Hg were found in the liver; the co-treatment did not alter this parameter. EWH prevented the morphological and metabolic disorder induced by Hg, by improving antioxidant defenses, inactivating pro-apoptotic pathways and normalizing the mRNA expression of PPARs and adipokines. Its effects enabled an increase in insulin levels and a normal balance between the fat storage and expenditure mechanisms in WAT. : EWH may have potential benefits in the prevention and management of Hg-related metabolic disorders.
为了研究蛋清水解物（EWH）对汞（Hg）引起的白色脂肪组织（WAT）功能障碍中脂质和血糖代谢紊乱的影响。将 Wistar 大鼠处理 60 天：对照组（生理盐水，肌肉内 - i.m.）；水解物（EWH，灌胃，1 g/kg/天）；汞（HgCl，肌肉内，1 剂量 4.6μg/kg，随后剂量 0.07μg/kg/天）和水解物-汞（EWH-HgCl）。在附睾白色脂肪组织（eWAT）、胰腺和肝脏中进行 Hg 水平和组织学分析。在 eWAT 中分析 GRP78、CHOP、PPARα、PPARγ、瘦素、脂联素和 CD11mRNA 的表达。测量血浆脂质谱、血糖和胰岛素水平。还评估了血浆和肝脏中的抗氧化状态。EWH 摄入可防止 Hg 暴露引起的 eWAT 重量、脂肪细胞大小、胰岛素水平和抗氧化防御降低，以及葡萄糖和甘油三酯水平升高；与 EWH 共同治疗的大鼠肝谷胱甘肽水平较高。Hg 诱导的 CHOP、PPARα 和瘦素 mRNA 表达增加在共同治疗的大鼠中减少。EWH 并未改变 Hg 处理大鼠中 GRP78、PPARγ 和脂联素的 mRNA 表达升高。在肝脏中发现 Hg 水平升高；共同处理并未改变该参数。EWH 通过改善抗氧化防御、激活凋亡途径和使 PPARs 和脂肪因子的 mRNA 表达正常化，防止 Hg 引起的形态和代谢紊乱。它的作用使胰岛素水平升高，并使 WAT 中脂肪储存和消耗机制之间达到正常平衡。EWH 可能对预防和管理与 Hg 相关的代谢紊乱具有潜在益处。
