Escobar Alyne Goulart, Rizzetti Danize Aparecida, Piagette Janaina Trindade, Peçanha Franck Maciel, Vassallo Dalton Valentim, Miguel Marta, Wiggers Giulia Alessandra
Graduate Program in Biochemistry and Multicentric Graduate Program in Physiological Sciences, Universidade Federal do Pampa, Uruguaiana, Brazil.
Department of Physiological Sciences, Universidade Federal do Espírito Santo and School of Medicine of Santa Casa de Misericórdia (EMESCAM), Vitória, Brazil.
Front Physiol. 2020 Nov 20;11:595767. doi: 10.3389/fphys.2020.595767. eCollection 2020.
We investigated the antioxidant protective power of egg white hydrolysate (EWH) against the vascular damage induced by mercury chloride (HgCl) exposure in resistance arteries. Male Wistar rats received for 60 days: (I) intramuscular injections (i.m.) of saline and tap water by gavage - Untreated group; (II) 4.6 μg/kg of HgCl i.m. for the first dose and subsequent doses of 0.07 μg/kg/day and tap water by gavage - HgCl group; (III) saline i.m. and 1 g/kg/day of EWH by gavage - EWH group, or (IV) the combination of the HgCl i.m. and EWH by gavage - EWH + HgCl group. Blood pressure (BP) was indirectly measured and dose-response curves to acetylcholine (ACh), sodium nitroprusside (SNP), and noradrenaline (NE) were assessed in mesenteric resistance arteries (MRA), as production of superoxide anion, nitric oxide (NO) release, vascular reactive oxygen species (ROS), lipid peroxidation, and antioxidant status. Egg white hydrolysate prevented the elevation in BP and the vascular dysfunction after HgCl exposure; restored the NO-mediated endothelial modulation and inhibited the oxidative stress and inflammatory pathways induced by HgCl. Egg white hydrolysate seems to be a useful functional food to prevent HgCl-induced vascular toxic effects in MRA.
我们研究了蛋清水解物（EWH）对氯化汞（HgCl）暴露诱导的阻力动脉血管损伤的抗氧化保护作用。雄性Wistar大鼠接受60天的处理：（I）肌肉注射生理盐水并经口灌胃自来水——未处理组；（II）首次剂量为4.6μg/kg的HgCl肌肉注射，随后剂量为0.07μg/kg/天，并经口灌胃自来水——HgCl组；（III）肌肉注射生理盐水并经口灌胃1g/kg/天的EWH——EWH组，或（IV）肌肉注射HgCl并经口灌胃EWH——EWH + HgCl组。间接测量血压（BP），并在肠系膜阻力动脉（MRA）中评估对乙酰胆碱（ACh）、硝普钠（SNP）和去甲肾上腺素（NE）的剂量反应曲线，以及超氧阴离子的产生、一氧化氮（NO）释放、血管活性氧（ROS）、脂质过氧化和抗氧化状态。蛋清水解物可预防HgCl暴露后血压升高和血管功能障碍；恢复NO介导的内皮调节，并抑制HgCl诱导的氧化应激和炎症途径。蛋清水解物似乎是一种有用的功能性食品，可预防HgCl对MRA诱导的血管毒性作用。
