Key Laboratory of Flexible Electronics (KLOFE) &, Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), Nanjing, 211800, China.
Frontiers Science Center for Flexible Electronics, Xi'an Institute of Flexible Electronics (IFE) and, Xi'an Institute of Biomedical Materials & Engineering, Northwestern Polytechnical University, Xi'an, 710072, China.
Chembiochem. 2022 Jul 19;23(14):e202200217. doi: 10.1002/cbic.202200217. Epub 2022 May 30.
Impaired mitophagy hinders the clearance of damaged mitochondria, inducing pathological states. Knowledge of this phenomenon is key to diagnosing certain diseases and understanding their pathogenesis. Mitophagy involves an acidization process that could serve as an ideal detection target. In this work, we designed and synthesized a mitochondrial-targeting fluorescence probe, Z2, for evaluating pH variation. This probe exhibited remarkable "turn-on" fluorescence under acidic conditions. In biological applications, Z2 showed a strong, specific pH detection capacity in Parkin-overexpressing HeLa cells during the mitophagy process. The "turn-on" fluorescence property of Z2 was also used to detect pH variations in Caenorhabditis elegans. This probe, as a novel pH assessment tool, may facilitate further research of mitophagy-associated pathological patterns.
线粒体自噬受损会阻碍受损线粒体的清除，从而导致病理状态。了解这一现象是诊断某些疾病和理解其发病机制的关键。线粒体自噬涉及酸化过程，这可以作为一个理想的检测靶点。在这项工作中，我们设计并合成了一种线粒体靶向荧光探针 Z2，用于评估 pH 值的变化。该探针在酸性条件下表现出显著的“开启”荧光。在生物学应用中，Z2 在 Parkin 过表达的 HeLa 细胞的线粒体自噬过程中表现出很强的、特异性的 pH 值检测能力。Z2 的“开启”荧光特性也被用于检测秀丽隐杆线虫中的 pH 值变化。作为一种新的 pH 值评估工具，该探针可能有助于进一步研究与线粒体自噬相关的病理模式。
