Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Bioresour Technol. 2022 Jul;356:127313. doi: 10.1016/j.biortech.2022.127313. Epub 2022 May 13.
The actinomycetes strain Streptomyces sp. XD-11-9-3 and Streptomyces sp. 5 were isolated and presented poor denitrification performance. Co-culture of actinomycetes triggers nitrogen removal capacity under aerobic conditions (reduced 96% of total nitrogen). Nitrogen balance analysis presented that 71% of initial nitrogen converted as gaseous nitrogen. Moreover, co-culture increased the concentrations of adenosine triphosphate (>2.1 folds) and electron-transmission system activity (>1.5 folds) significantly. The co-culture presented excellent carbon source metabolism activity (especially amines and carboxylic acids) compared with monoculture. The removal efficiency of total nitrogen in the micro-polluted landscape water water reached 61% in the co-culture system, and the algal survival could be inhibited significantly. However, the dominant niche of the co-culture system restrained the diversity of the indigenous nirS-type denitrifying bacterial community. This study provided a novel pathway to the research of co-culture inefficiency aerobic denitrifier and further application in the restoration of polluted water.
放线菌菌株 Streptomyces sp. XD-11-9-3 和 Streptomyces sp. 5 被分离出来，表现出较差的反硝化性能。放线菌的共培养在有氧条件下触发了脱氮能力（总氮减少了 96%）。氮平衡分析表明，71%的初始氮转化为气态氮。此外，共培养显著增加了三磷酸腺苷（>2.1 倍）和电子传递系统活性（>1.5 倍）的浓度。与单培养相比，共培养表现出优异的碳源代谢活性（尤其是胺类和羧酸类）。在微污染景观水中，共培养系统对总氮的去除率达到 61%，并能显著抑制藻类的生存。然而，共培养系统的优势小生境限制了土著 nirS 型反硝化细菌群落的多样性。本研究为共培养低效好氧反硝化菌的研究提供了一条新途径，并进一步应用于污染水的修复。
