School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China.
School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China.
Bioresour Technol. 2022 Jul;356:127287. doi: 10.1016/j.biortech.2022.127287. Epub 2022 May 13.
Anammox and sulfide-dependent autotrophic denitrification (ASDAD) coupling system can improve the nitrogen removal, but high sulfide concentration will affect the activity of anaerobic ammonia-oxidizing bacteria (AnAOB). Gel immobilization technology can enhance the survivability of microorganisms in unsuitable environments. Therefore, in this investigation, gel immobilization technology was applied into the ASDAD coupling system to explore the removal performance and microbial communities. The results showed that the optimal S/NO was 0.6, under which the best TN removal efficiency was 85.69%. The removal performance of ASDAD coupling system was stable under rapid variations of nitrogen loading rate and sulfide loading rate. Immobilized sludge cubes could attenuate the effects of temperature on AnAOB and sulfide-oxidizing bacteria. Observations of SEM and stereoscope suggested that AnAOB was more likely to exist on the surface of the sludge cubes. Thiobacillus, Candidatus Brocadia, and Candidatus Kuenenia were the main functional bacteria in the coupling system.
厌氧氨氧化和硫化物依赖型自养反硝化（ASDAD）耦合系统可以提高氮的去除率，但高浓度的硫化物会影响厌氧氨氧化菌（AnAOB）的活性。凝胶固定化技术可以增强微生物在不适宜环境中的生存能力。因此，在本研究中，凝胶固定化技术被应用于 ASDAD 耦合系统，以探索其去除性能和微生物群落。结果表明，最佳 S/NO 为 0.6，在此条件下，TN 的去除效率最佳为 85.69%。ASDAD 耦合系统在氮负荷和硫负荷快速变化下的去除性能稳定。固定化污泥块可以减轻温度对 AnAOB 和硫氧化菌的影响。SEM 和立体显微镜观察表明，AnAOB 更可能存在于污泥块的表面。硫杆菌属、Brocadia 菌属和 Kuenenia 菌属是耦合系统中的主要功能菌。
