Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou, People's Republic of China.
Environ Technol. 2024 May;45(12):2427-2437. doi: 10.1080/09593330.2023.2174048. Epub 2023 Feb 14.
Anammox is one of the most innovative nitrogen removal technologies, while its functional bacteria-anaerobic ammonia-oxidizing bacteria (AAOB) is sensitive to the impurities in the wastewater. In this study, the long-term effects of sulfide at different concentrations (0, 5, 10, 20, 30, 50, 25 mg L) on low substrate Anammox process were studied. The results showed that when the sulfide was 25-30 mg L, AAOB was well coupled with sulfide-denitrifying bacteria and the total nitrogen removal efficiency (TNRE) reached a maximum of 91.0%. The hydroxylamine oxidoreductase activity and Heme-c reached 1.678 EU g SS and 0.0023 mmol g SS, respectively, with the and gene concentrations as 2.52 × 10 and 4.45 × 10 copies mL. 50 mg L sulfide inhibited the nitrogen removal by AAOB, resulting in the TNRE decreasing to 81.7%. The experimental results provide a reference for the practical application of Anammox in treating sulfur-containing wastewater.
厌氧氨氧化是最具创新性的氮去除技术之一，但其功能菌——厌氧氨氧化菌（AAOB）对废水中的杂质敏感。本研究在不同浓度（0、5、10、20、30、50、25mg/L）的硫化物作用下，考察了其对低基质厌氧氨氧化工艺的长期影响。结果表明，当硫化物浓度为 25-30mg/L 时，AAOB 与硫-反硝化菌良好耦合，总氮去除效率（TNRE）达到最大值 91.0%。羟胺氧化还原酶活性和 Heme-c 分别达到 1.678EU g SS 和 0.0023mmol g SS， 基因和 基因浓度分别为 2.52×10 和 4.45×10 拷贝/mL。50mg/L 硫化物抑制了 AAOB 的脱氮作用，导致 TNRE 降低至 81.7%。实验结果为厌氧氨氧化在处理含硫废水方面的实际应用提供了参考。
