Fujii Seed Co. Ltd., Fujii Seed, 2-12-38 Juso-higashi, Yodogawa-ku, Osaka, 532-0023, Japan.
Kazusa DNA Research Institute, Kisarazu, Chiba, 292-0818, Japan.
Sci Rep. 2022 May 16;12(1):8063. doi: 10.1038/s41598-022-11544-7.
Carrot is a major source of provitamin A in a human diet. Two of the most important traits for carrot breeding are carotenoid contents and root color. To examine genomic regions related to these traits and develop DNA markers for carrot breeding, we performed an association analysis based on a general liner model using genome-wide single nucleotide polymorphism (SNPs) in two F populations, both derived from crosses of orange root carrots bred in Japan. The analysis revealed 21 significant quantitative trait loci (QTLs). To validate the detection of the QTLs, we also performed a QTL analysis based on a composite interval mapping of these populations and detected 32 QTLs. Eleven of the QTLs were detected by both the association and QTL analyses. The physical position of some QTLs suggested two possible candidate genes, an Orange (Or) gene for visual color evaluation, and the α- and β-carotene contents and a chromoplast-specific lycopene β-cyclase (CYC-B) gene for the β/α carotene ratio. A KASP marker developed on the Or distinguished a quantitative color difference in a different, related breeding line. The detected QTLs and the DNA marker will contribute to carrot breeding and the understanding of carotenoid biosynthesis and accumulation in orange carrots.
胡萝卜是人类饮食中维生素 A 的主要来源。胡萝卜育种的两个最重要的特征是类胡萝卜素含量和根色。为了研究与这些特征相关的基因组区域，并为胡萝卜育种开发 DNA 标记，我们使用两个源自日本橙色根胡萝卜杂交的 F2 群体的全基因组单核苷酸多态性（SNP），基于广义线性模型进行了关联分析。该分析揭示了 21 个显著的数量性状位点（QTL）。为了验证 QTL 的检测，我们还对这些群体进行了基于复合区间作图的 QTL 分析，检测到 32 个 QTL。关联和 QTL 分析都检测到 11 个 QTL。一些 QTL 的物理位置提示了两个可能的候选基因，一个用于视觉颜色评估的橙色（Or）基因，以及用于 β/α 类胡萝卜素比值的 α-和 β-胡萝卜素含量和质体特异性番茄红素 β-环化酶（CYC-B）基因。在一个不同的相关育种系上，基于 Or 开发的 KASP 标记区分了定量颜色差异。检测到的 QTL 和 DNA 标记将有助于胡萝卜育种以及对橙色胡萝卜中类胡萝卜素生物合成和积累的理解。
