Department of Microbiology, Lahijan Branch, Islamic Azad University (IAU), Lahijan, Guilan, Iran.
Department of Biology, University of Guilan, Rasht, Iran.
Arch Microbiol. 2022 May 16;204(6):329. doi: 10.1007/s00203-022-02930-w.
Biofilm formation is an important factor in disease development by Pseudomonas aeruginosa. Similar to many bacterial species, biofilm formation in P. aeruginosa is regulated by the bacterial quorum sensing system. The pel genes are responsible for the synthesis of a glucose-rich polysaccharide that is associated with biofilm initiation and maturation. The antibiofilm potential of ibuprofen has been reported; however, the effect of the drug on the expression of the genes involved with biofilm formation has rarely been described. In this work, the effect of ibuprofen on the biofilm formation and expression of pelD and pelF genes among pathogenic P. aeruginosa strains was investigated. Multiple drug-resistant P. aeruginosa strains were treated with ibuprofen at ½ MIC concentration and their biofilm formation and expression of pelD and pelF genes was determined using the crystal violet and real-time PCR assays, respectively. The results showed that the ibuprofen at 1024 µg/mL significantly reduced biofilm formation of P. aeruginosa strains by 52-77%, compared with the controls. In addition, treating the bacteria with ibuprofen decreased the expression of pelD and pelF genes to 0.56 and 0.69 folds, respectively. We hypothesized that the attenuation of the pel genes could be associated with the reduction of bacterial QS autoinducers, which in turn reduced cellular c-di-GMP level. This work suggests that ibuprofen is a potent antibiofilm drug that could be used to enhance bacterial susceptibility to antimicrobials through the inhibition of biofilm formation.
生物膜的形成是铜绿假单胞菌引发疾病的一个重要因素。与许多细菌物种相似，铜绿假单胞菌生物膜的形成受细菌群体感应系统的调节。pel 基因负责合成与生物膜起始和成熟相关的富含葡萄糖的多糖。布洛芬具有抗生物膜的潜力；然而，该药物对生物膜形成相关基因表达的影响很少被描述。在这项工作中，研究了布洛芬对致病性铜绿假单胞菌菌株生物膜形成和 pelD 和 pelF 基因表达的影响。用布洛芬（浓度为半最小抑菌浓度的 1/2）处理多重耐药铜绿假单胞菌菌株，并分别使用结晶紫和实时 PCR 测定法测定其生物膜形成和 pelD 和 pelF 基因的表达。结果表明，与对照组相比，1024µg/mL 的布洛芬显著降低了铜绿假单胞菌菌株的生物膜形成率，降低了 52-77%。此外，用布洛芬处理细菌可使 pelD 和 pelF 基因的表达分别降低至 0.56 和 0.69 倍。我们假设 pel 基因的衰减可能与细菌 QS 自动诱导物的减少有关，进而降低了细胞 c-di-GMP 水平。这项工作表明，布洛芬是一种有效的抗生物膜药物，可通过抑制生物膜形成来增强细菌对抗微生物药物的敏感性。
