Department of Zoology, Faculty of Science, Cairo University, Giza 12613, Egypt.
Curr Drug Deliv. 2023;20(5):575-586. doi: 10.2174/1567201819666220509161548.
Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
The present study aimed to develop nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as an alternative cancer remedy, and then the evaluated its biological activities.
The PGPs yield and chemical composition were evaluated, and then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase. The physicochemical characterization of PGPs-NE was then analyzed. The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
The PGPs yield was 10%. The total sugar and protein content of PGPs was 44.66 mg/dl and 19.83μg/ml, respectively. PGPs were mainly composed of five monosaccharides including fructose, glucose, galactose, rhamnose, and arabinose. Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204, and 207nm and a transmittance of 80%. Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV). The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm. Additionally, the method used to prepare the PGPs-NE formulation provided high entrapment efficiency (92.82%). The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs.
These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.
结直肠癌是非洲最严重的胃肠道癌症之一，其预防是当代全球医学面临的一个重大挑战。
本研究旨在开发使用石榴多糖（PGPs）作为替代癌症治疗方法的纳米乳液药物传递系统，然后评估其生物学活性。
评估 PGPs 的产率和化学成分，然后使用油相的自乳化技术制备 PGPs 纳米乳液（PGPs-NE）。分析 PGPs-NE 的物理化学特性。还评估了 PGPs 和 PGPs-NE 的体外抗氧化、抗炎和抗肿瘤活性。
PGPs 的产率为 10%。PGPs 的总糖和蛋白质含量分别为 44.66mg/dl 和 19.83μg/ml。PGPs 主要由五种单糖组成，包括果糖、葡萄糖、半乳糖、鼠李糖和阿拉伯糖。关于物理化学特性，所制备的 PGPs-NE 在 202、204 和 207nm 处有三个光吸收带，透光率为 80%。其平均水动力粒径为 9.5nm，PDI 小于 0.2，zeta 电位为负（-30.6 mV）。透射电子显微镜研究证实了 PGPs-NE 的球形形状，平均粒径小于 50nm。此外，用于制备 PGPs-NE 制剂的方法提供了高包封效率（92.82%）。本研究表明，与游离 PGPs 相比，PGPs-NE 具有更强的抗氧化、抗炎和抗肿瘤作用。
这些有前途的研究结果为新型 PGPs-NE 作为 CRC 替代治疗方法的可能疗效提供了证据。
