Bi Hongxia, Deng Rong, Liu Yanbin
Center of Infectious Disease, West China Hospital, Sichuan University, Chengdu, China.
Acta Microbiol Immunol Hung. 2022 May 17. doi: 10.1556/030.2022.01689.
The ica gene of Staphylococcus aureus (S. aureus) plays a vital role in its growth and biofilm formation. Among them, IcaA and IcaB are critical proteins for synthesizing extracellular polysaccharides and biofilms in S. aureus. To investigate whether the formation of S. aureus biofilms can be inhibited through the IcaA and IcaB proteins by the presence of linezolid.
The icaA and icaB genes of S. aureus ATCC 25923 were silenced by homologous recombination. The critical roles of icaA and icaB in S. aureus were analysed by observing the growth curve and biofilm formation after linezolid treatment. Then, the effect of linezolid on the morphology of S. aureus was observed by scanning electron microscopy. Finally, the potential binding ability of linezolid to Ica proteins was predicted by molecular docking.
The icaA- and icaB-silenced strains were successfully constructed, and the sensitivity of S. aureus to linezolid was decreased after icaA and icaB silencing. Scanning electron microscopy showed that linezolid caused invagination of the S. aureus surface and reduced the production of biofilms. Molecular docking results showed that linezolid could bind to IcaA and IcaB proteins.
IcaA and IcaB are potential targets of linezolid in inhibiting the biofilm formation of S. aureus (ATCC 25923).
金黄色葡萄球菌的ica基因在其生长和生物膜形成中起着至关重要的作用。其中，IcaA和IcaB是金黄色葡萄球菌合成细胞外多糖和生物膜的关键蛋白。旨在研究利奈唑胺的存在是否能通过IcaA和IcaB蛋白抑制金黄色葡萄球菌生物膜的形成。
通过同源重组使金黄色葡萄球菌ATCC 25923的icaA和icaB基因沉默。通过观察利奈唑胺处理后的生长曲线和生物膜形成情况，分析icaA和icaB在金黄色葡萄球菌中的关键作用。然后，通过扫描电子显微镜观察利奈唑胺对金黄色葡萄球菌形态的影响。最后，通过分子对接预测利奈唑胺与Ica蛋白的潜在结合能力。
成功构建了icaA和icaB基因沉默菌株，icaA和icaB基因沉默后金黄色葡萄球菌对利奈唑胺的敏感性降低。扫描电子显微镜显示，利奈唑胺导致金黄色葡萄球菌表面内陷并减少生物膜的产生。分子对接结果表明，利奈唑胺可与IcaA和IcaB蛋白结合。
IcaA和IcaB是利奈唑胺抑制金黄色葡萄球菌（ATCC 25923）生物膜形成的潜在靶点。
