Lee Soomin, Kim Sejeong, Lee Heeyoung, Ha Jimyeong, Lee Jeeyeon, Choi Yukyung, Oh Hyemin, Yoon Yohan, Choi Kyoung-Hee
1 Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea.
2 Department of Oral Microbiology, College of Dentistry, Wonkwang University, Iksan, Jeonbuk 54896, Korea.
J Food Prot. 2018 Mar;81(3):412-416. doi: 10.4315/0362-028X.JFP-17-238.
The objective of this study was to elucidate the role of the icaA gene in biofilm formation of Staphylococcus aureus exposed to NaCl. The icaA-deletion mutant of S. aureus ATCC 13565 was constructed with the temperature-sensitive plasmid pIMAY. Microtiter plate assays were performed to confirm biofilm formation for both the wild type and the mutant at 0% (control), 2, 4, and 6% NaCl. The microtiter plate assay revealed that biofilm formation by the wild type increased ( P < 0.05) as NaCl concentration increased, but biofilm formation of the mutant was not affected by NaCl concentration. Biofilm formation by the mutant was lower ( P < 0.05) than that by the wild type. These results indicate that icaA plays an important role in biofilm formation by S. aureus when the pathogen is exposed to NaCl.
本研究的目的是阐明icaA基因在暴露于氯化钠的金黄色葡萄球菌生物膜形成中的作用。使用温度敏感质粒pIMAY构建了金黄色葡萄球菌ATCC 13565的icaA缺失突变体。进行了微量滴定板试验，以确认野生型和突变体在0%（对照）、2%、4%和6%氯化钠条件下的生物膜形成情况。微量滴定板试验显示，野生型的生物膜形成随着氯化钠浓度的增加而增加（P<0.05），但突变体的生物膜形成不受氯化钠浓度的影响。突变体的生物膜形成低于野生型（P<0.05）。这些结果表明，当病原体暴露于氯化钠时，icaA在金黄色葡萄球菌的生物膜形成中起重要作用。
