从鸭疫里默氏菌中分离到一种新型 A 类扩展谱β-内酰胺酶 RASA-1 的特性研究。
Characterization of RASA-1, a novel class A extended-spectrum beta-lactamase from Riemerella anatipestifer.
机构信息
College of Veterinary Medicine, Southwest University, Beibei, Chongqing, China.
Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
出版信息
Vet Microbiol. 2022 Jul;270:109456. doi: 10.1016/j.vetmic.2022.109456. Epub 2022 May 12.
A novel chromosomally-located β-lactamase gene, bla, was identified in Riemerella anatipestifer RA-CH-1. The RASA-1, encoded by bla, was a class A extended-spectrum β-lactamase (ESBL), which shared 42.7% and 40.5% identities with the RAA-1 and CGA-1 β-lactamase, respectively. Overexpression of RASA-1 in Escherichia coli confers broad resistance to β-lactams and the purified native RASA-1 revealed ESBL-like hydrolysis activity. Blasting in GenBank showed that bla was exclusively detected in Riemerella anatipestifer. Moreover, sequence analysis revealed that this gene was located within the multi-resistance region of Riemerella anatipestifer genome.
在鸭疫里默氏杆菌 RA-CH-1 中发现了一个新型的位于染色体上的β-内酰胺酶基因 bla。由 bla 编码的 RASA-1 是一种 A 类扩展谱β-内酰胺酶（ESBL），分别与 RAA-1 和 CGA-1 β-内酰胺酶具有 42.7%和 40.5%的同一性。RASA-1 在大肠杆菌中的过表达赋予了对β-内酰胺类抗生素的广泛抗性，并且纯化的天然 RASA-1 显示出 ESBL 样的水解活性。在 GenBank 中的 Blast 显示 bla 仅在鸭疫里默氏杆菌中被检测到。此外，序列分析表明，该基因位于鸭疫里默氏杆菌基因组的多耐药区域内。
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