Department of Chemical Engineering and Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu, Taiwan, ROC.
Department of Chemical Engineering and Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu, Taiwan, ROC; Department of Orthopaedic Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan, ROC.
Biomaterials. 2022 Jul;286:121574. doi: 10.1016/j.biomaterials.2022.121574. Epub 2022 May 10.
The reconstruction of a large bone defect to an extent that exceeds its self-healing capacity has been a great clinical challenge. In pursuit of this goal, a biomaterial-based scaffold that comprises radially aligned mineralized collagen (RA-MC) fibers that incorporate nanosilicon (RA-MC/nSi), is proposed. The chemical composition of the MC fibers is similar to that of natural bone matrices. The therapeutic efficacy of the RA-MC/nSi scaffold is evaluated in a mouse model with an experimentally created large calvarial defect. In vitro and in vivo results reveal that the RA-MC fibers of the scaffold guide the directional infiltration and migration of reparative cells from the host tissue toward the center of the defect, suggesting a potential application in promoting osteoconductivity. The incorporated nSi renders the scaffold able sustainably to release gaseous hydrogen and water-soluble silicic acid during the healing process. The released hydrogen gas can effectively regulate redox homeostasis and mitigate excessive inflammation, and the silicic acid can promote the proliferation of reparative cells and enhance their osteogenic differentiation, indicative of osteoinductivity. These findings support the use of the as-proposed biomimetic RA-MC/nSi scaffold as a promising bone substitute to enhance the regeneration of large bone defects.
重建超出其自身愈合能力的大骨缺损一直是一个巨大的临床挑战。为了实现这一目标，提出了一种基于生物材料的支架，该支架包含径向排列的矿化胶原（RA-MC）纤维，其中掺入纳米硅（RA-MC/nSi）。MC 纤维的化学成分与天然骨基质相似。在具有实验性大颅骨缺损的小鼠模型中评估了 RA-MC/nSi 支架的治疗效果。体外和体内结果表明，支架的 RA-MC 纤维引导修复细胞从宿主组织向缺损中心的定向渗透和迁移，表明其在促进成骨能力方面具有潜在应用。掺入的 nSi 使支架能够在愈合过程中持续释放气态氢和水溶性硅酸。释放的氢气可以有效调节氧化还原平衡并减轻过度炎症，硅酸可以促进修复细胞的增殖并增强其成骨分化，表明具有成骨诱导性。这些发现支持使用所提出的仿生 RA-MC/nSi 支架作为有前途的骨替代物来增强大骨缺损的再生。
