Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, 796014, Mizoram, India.
Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, 796014, Mizoram, India.
Microb Pathog. 2022 Jun;167:105575. doi: 10.1016/j.micpath.2022.105575. Epub 2022 May 14.
The present investigation was undertaken to map the distribution of Anapalsma species infection in cattle from the Aizawl region of Mizoram, India, in relation to various risk factors, and to study the haemato-biochemical alterations, oxidant/antioxidant status and serum trace mineral levels in cattle with naturally occurring Anapalsma marginale infection. The study was carried out over 31 months from June 2019 to December 2021. A total of 401 cattle blood samples were collected and screened for the presence of Anaplasma spp. by microscopic examination and polymerase chain reaction (PCR). Non-infected clinically healthy cattle (n = 21) served as control. Blood samples were collected to study the haemogram and serum samples were used for the evaluation of biochemical parameters, oxidative stress indices and trace minerals. During the study period, an overall prevalence of 15.71% was recorded for A. marginale infection in cattle. The prevalence of A. marginale infection was highly associated with age, sex, breed and tick infestation status of animals, floor system and management of farms, and season. The mean values of total erythrocyte count (TEC), haemoglobin (Hb), packed cell volume (PCV), total platelet count, total protein, albumin, superoxide dismutase (SOD), glutathione (GSH), total antioxidant capacity (TAC), copper (Cu) and zinc (Zn) were significantly (P < 0.05) lower, whereas the mean values of mean corpuscular volume (MCV), aspartate aminotransferase (AST), total bilirubin, indirect bilirubin, blood urea nitrogen (BUN), creatinine and lipid hydroperoxide (LPO) were significantly (P < 0.05) higher in cattle infected with A. marginale. A negative correlation of TEC with LPO, and a positive correlation with SOD, GSH, TAC, Cu and Zn suggest a possible link between oxidative stress and the haemolytic crisis noticed in bovine anaplasmosis. Incorporation of antioxidants and organ protective drugs as an adjunct therapy may result in better prognosis.
本研究旨在绘制印度米佐拉姆邦 Aizawl 地区牛感染 Anaplasma 种的分布情况，以及与各种风险因素的关系，并研究自然发生的 Anaplasma marginale 感染牛的血液生化学变化、氧化应激状态和血清痕量矿物质水平。研究于 2019 年 6 月至 2021 年 12 月进行了 31 个月。共采集了 401 份牛血样，通过显微镜检查和聚合酶链反应（PCR）筛查 Anaplasma spp。非感染的临床健康牛（n=21）作为对照。采集血样研究血象，采集血清样评估生化参数、氧化应激指标和痕量矿物质。在研究期间，牛的 A. marginale 感染总流行率为 15.71%。A. marginale 感染的流行与动物的年龄、性别、品种和蜱虫感染状况、地板系统和农场管理以及季节高度相关。总红细胞计数（TEC）、血红蛋白（Hb）、红细胞压积（PCV）、血小板总数、总蛋白、白蛋白、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、总抗氧化能力（TAC）、铜（Cu）和锌（Zn）的平均值明显（P<0.05）降低，而平均红细胞体积（MCV）、天冬氨酸转氨酶（AST）、总胆红素、间接胆红素、血尿素氮（BUN）、肌酐和脂质过氧化物（LPO）的平均值明显（P<0.05）升高在感染 A. marginale 的牛中。TEC 与 LPO 呈负相关，与 SOD、GSH、TAC、Cu 和 Zn 呈正相关，提示氧化应激与牛边虫病中观察到的溶血性危机之间可能存在联系。作为辅助治疗加入抗氧化剂和器官保护药物可能会有更好的预后。
