多样化的英文关键词搜索揭示了 scriptaid 处理对体细胞核移植后猪胚胎发育的价值。
A diverse English keyword search reveals the value of scriptaid treatment for porcine embryo development following somatic cell nuclear transfer.
机构信息
Northeast Agricultural University, College of Arts and Sciences, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, P. R. China.
Heilongjiang Academy of Agricultural Sciences, Animal Husbandry Research Institute, No. 368 Xuefu Road, Harbin 150086, P. R. China.
出版信息
Reprod Fertil Dev. 2022 Jul;34(11):798-803. doi: 10.1071/RD22025.
CONTEXT
Incomplete epigenetic reprogramming of histone deacetylation (HDAC) is one of the main reasons for the low efficiency of somatic cell nuclear transfer (SCNT). Scriptaid is a synthetic HDAC inhibitor (HDACi) that may improve the efficiency of porcine SCNT.
AIMS
This study aimed to determine whether scriptaid increases the number of blastocyst cells or the cleavage rate.
METHODS
We conducted a meta-analysis of the pertinent literature published over the past decade.
KEY RESULTS
A total of 73 relevant papers were retrieved using a diverse English keyword search, and 11 articles were used for the meta-analysis. Scriptaid was positively correlated with blastocyst rate but had no effect on cleavage rate or blastocyst cell number. A subgroup analysis of blastocyst cell number showed that the staining method was the source of the heterogeneity.
CONCLUSIONS
In SCNT embryos, scriptaid treatment after activation can promote embryonic development, but there may be adverse effects on early development.
IMPLICATIONS
HDACi research should focus on SCNT birth efficiency.
背景
组蛋白去乙酰化（HDAC）的不完全表观遗传重编程是体细胞细胞核移植（SCNT）效率低的主要原因之一。Scriptaid 是一种合成的 HDAC 抑制剂（HDACi），可能会提高猪 SCNT 的效率。
目的
本研究旨在确定 Scriptaid 是否会增加囊胚细胞数量或提高卵裂率。
方法
我们对过去十年发表的相关文献进行了荟萃分析。
主要结果
使用多种英文关键词搜索共检索到 73 篇相关文献，其中 11 篇文章用于荟萃分析。Scriptaid 与囊胚率呈正相关，但对卵裂率或囊胚细胞数量没有影响。囊胚细胞数量的亚组分析表明，染色方法是异质性的来源。
结论
在 SCNT 胚胎中，激活后使用 Scriptaid 处理可以促进胚胎发育，但可能对早期发育有不良影响。
意义
HDACi 的研究应侧重于 SCNT 的出生率。
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