Department of Plastic Surgery, First Affiliated Hospital, Bengbu Medical College, 287 Changhuai Road, Bengbu 233004, China; The School of Life Sciences, Bengbu Medical College, 2600 Donghai Road, Bengbu 233030, China.
Department of Orthopaedics, First Affiliated Hospital, Bengbu Medical College, 287 Changhuai Road, Bengbu 233004, China.
Biomater Adv. 2022 Mar;134:112700. doi: 10.1016/j.msec.2022.112700. Epub 2022 Feb 7.
High-efficiency repair of critical bone defects is a pressing problem in clinical practice. However, most biological replacement materials do not simultaneously satisfy the dual requirements of mechanical strength and cell compatibility. In this study, chitosan methacryloyl (CSMA) and β-tricalcium phosphate (β-TCP) were subjected to photo-crosslinking to form the CSMA/β-TCP composite hydrogel, which has strong mechanical properties contributing to bone regeneration. In addition, its scaffold can alter the morphology of bone marrow mesenchymal stem cells (BMSCs), promote their proliferation, enhance the expression of alkaline phosphatase (ALP), and augment the nodular deposition of calcium. Meanwhile, the expressions of osteogenic proteins (ALP, osteocalcin, and osteopontin) were upregulated and the regulatory mechanism of the Hippo signaling pathway was verified. Moreover, animal experiments have confirmed that CSMA/β-TCP has adequate biocompatibility and bone regeneration. These results demonstrate the immense potential of the CSMA/β-TCP composite hydrogel in bone regeneration therapy.
高效修复临界骨缺损是临床实践中的一个紧迫问题。然而，大多数生物替代材料不能同时满足机械强度和细胞相容性的双重要求。在本研究中，壳聚糖甲基丙烯酰基（CSMA）和β-磷酸三钙（β-TCP）经光交联形成 CSMA/β-TCP 复合水凝胶，具有较强的机械性能，有助于骨再生。此外，其支架可以改变骨髓间充质干细胞（BMSCs）的形态，促进其增殖，增强碱性磷酸酶（ALP）的表达，并增加钙的结节沉积。同时，上调成骨蛋白（ALP、骨钙素和骨桥蛋白）的表达，并验证 Hippo 信号通路的调控机制。此外，动物实验证实 CSMA/β-TCP 具有足够的生物相容性和骨再生能力。这些结果表明 CSMA/β-TCP 复合水凝胶在骨再生治疗中具有巨大的潜力。
