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增加 miR-126 可通过调节 ZEB1 预防大鼠脑出血后脑损伤。

Increasing miR-126 Can Prevent Brain Injury after Intracerebral Hemorrhage in Rats by Regulating ZEB1.

机构信息

Departmennt of Neurosurgery, PuKou Branch Hospital of Jiangsu Province Hospital (Nanjing Pukou Central Hospital), Nanjing 211800, Jiangsu, China.

出版信息

Contrast Media Mol Imaging. 2022 Apr 30;2022:2698773. doi: 10.1155/2022/2698773. eCollection 2022.

DOI:10.1155/2022/2698773
PMID:35582235
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9078836/
Abstract

BACKGROUND

Studies have found that microRNA (miR) is abnormally expressed in intracerebral hemorrhage (ICH) and is considered a therapeutic target for ICH.

OBJECTIVE

To investigate the expression and role of miR-126 in the ICH rat model.

METHODS

The ICH rat model was established, and miR-126 agomir and ZEB1 antagomir were injected into the lateral ventricle of ICH rats. The neurological function and water content of brain tissue were evaluated 48 hours later. Brain tissue around the hematoma of rats was taken to detect the expression of miR-126, ZEB1, glial fibrillary acidic protein (GFAP), and inflammatory cytokines (TNF-, IL-1, and IL-6). The luciferase reporter gene was applied to analyze the relationship between miR-126 and ZEB1.

RESULTS

miR-126 was downregulated in the ICH rat model, while ZEB1 was upregulated. miR-126 agomir or ZEB1 antagomir injection could improve neurological function and cerebral edema in ICH rats. In addition, it could also reduce the expression of TNF-, IL-1, IL-6, and GFAP in the brain tissue of ICH rats. Luciferase reporter gene showed that ZEB1 could be targeted and regulated by miR-126.

CONCLUSION

miR-126 is downregulated in ICH rats, and miR-126 can reduce brain injury in ICH rats by inhibiting ZEB1 expression.

摘要

背景

研究发现，微小 RNA（miR）在脑出血（ICH）中异常表达，被认为是 ICH 的治疗靶点。

目的

探讨 miR-126 在 ICH 大鼠模型中的表达及作用。

方法

建立 ICH 大鼠模型，向 ICH 大鼠侧脑室注射 miR-126 激动剂和 ZEB1 拮抗剂。48 小时后评估大鼠的神经功能和脑组织含水量。取大鼠血肿周围脑组织，检测 miR-126、ZEB1、胶质纤维酸性蛋白（GFAP）和炎症细胞因子（TNF-α、IL-1、IL-6）的表达。应用荧光素酶报告基因分析 miR-126 与 ZEB1 的关系。

结果

ICH 大鼠模型中 miR-126 下调，ZEB1 上调。miR-126 激动剂或 ZEB1 拮抗剂注射可改善 ICH 大鼠的神经功能和脑水肿。此外，还可降低 ICH 大鼠脑组织中 TNF-α、IL-1、IL-6 和 GFAP 的表达。荧光素酶报告基因显示，ZEB1 可被 miR-126 靶向调控。

结论

miR-126 在 ICH 大鼠中下调，miR-126 通过抑制 ZEB1 表达减轻 ICH 大鼠脑损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce2/9078836/3aa91f4f964c/CMMI2022-2698773.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce2/9078836/2c2f812247e1/CMMI2022-2698773.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce2/9078836/124ea64cc834/CMMI2022-2698773.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce2/9078836/323ee89bb453/CMMI2022-2698773.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce2/9078836/3aa91f4f964c/CMMI2022-2698773.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce2/9078836/2c2f812247e1/CMMI2022-2698773.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce2/9078836/124ea64cc834/CMMI2022-2698773.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce2/9078836/323ee89bb453/CMMI2022-2698773.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce2/9078836/3aa91f4f964c/CMMI2022-2698773.004.jpg

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本文引用的文献

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2
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Oncol Lett. 2019 Sep;18(3):3274-3282. doi: 10.3892/ol.2019.10604. Epub 2019 Jul 11.
3
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J Physiol Biochem. 2024 May;80(2):277-286. doi: 10.1007/s13105-024-01017-y. Epub 2024 Mar 22.
4
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Biomark Res. 2024 Feb 2;12(1):17. doi: 10.1186/s40364-024-00568-y.
5
The role of the ZEB1-neuroinflammation axis in CNS disorders.ZEB1-神经炎症轴在中枢神经系统疾病中的作用。
J Neuroinflammation. 2022 Nov 19;19(1):275. doi: 10.1186/s12974-022-02636-2.
miR-126 通过调控 ZEB1 抑制宫颈癌的迁移和侵袭。
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4
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5
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J Biomed Inform. 2018 Jun;82:169-177. doi: 10.1016/j.jbi.2018.05.005. Epub 2018 May 12.
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