阿联酋阿布扎比酋长国单峰骆驼锥虫病诊断中显微镜检查、卡片凝集试验和实时荧光定量聚合酶链反应的比较

Comparison of Microscopy, Card Agglutination Test for , and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE.

作者信息

Habeeba Shameem, Khan Rashid Ali, Zackaria Hassan, Yammahi Saeed, Mohamed Zulaikha, Sobhi Wissam, AbdelKader Ayman, Alhosani Mohamed Ali, Muhairi Salama Al

机构信息

Microbiology and Molecular Biology Section, Veterinary Laboratories Division, Abu Dhabi, UAE.

Policy and Regulatory Division, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, UAE.

出版信息

J Vet Res. 2022 Mar 25;66(1):125-129. doi: 10.2478/jvetres-2022-0002. eCollection 2022 Mar.

DOI:10.2478/jvetres-2022-0002

PMID:35582483

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8959682/

Abstract

INTRODUCTION

Trypanosomosis is an important disease of dromedary camels caused by the pathogenic protozoan . This study aimed to compare three different tests for its diagnosis in this species: conventional microscopy, the card agglutination test for trypanosomosis/ (CATT/) and real-time PCR.

MATERIAL AND METHODS

Whole blood and serum samples collected from 77 dromedary camels of Abu Dhabi, United Arab Emirates, were analysed with the test methods stated. Statistical analysis was done using McNemar's chi-squared test, and Cohen's kappa index (κ) was calculated.

RESULTS

We obtained results with positivity of 18% (14/77) by microscopy, 22% by CATT (17/77) and 60% (46/77) by real-time PCR, with the chain reaction detecting at a respectively three- and two-fold greater rate than the other techniques. Analysis of the data revealed a relative sensitivity of 30.4% and 37.0% for microscopy and CATT, respectively, compared to real-time PCR. The difference between the real-time PCR's sensitivity and those of the other methods was statistically significant, with X values of 30.03 and 20.1, respectively (df = 1 and P = 0.05 in both cases). Agreement of microscopy results with those of with CATT was good (κ = 0.72; 95% CI = 0.62-0.82). Cohen's kappa index showed fair agreement of real-time PCR with microscopy (κ = 0.26; 95% CI = 0.16-0.36) whereas it was in poor agreement with CATT (κ = 0.09; 95% CI = 0.02-0.15).

CONCLUSION

Real-time PCR was found to be more sensitive than microscopy and CATT.

摘要

引言

锥虫病是由致病性原生动物引起的单峰骆驼的一种重要疾病。本研究旨在比较三种不同的检测方法对该物种锥虫病的诊断效果：传统显微镜检查、锥虫病卡片凝集试验（CATT）和实时荧光定量聚合酶链反应（PCR）。

材料与方法

对从阿拉伯联合酋长国阿布扎比的77峰单峰骆驼采集的全血和血清样本，采用上述检测方法进行分析。使用麦克尼马尔卡方检验进行统计分析，并计算科恩卡帕指数（κ）。

结果

显微镜检查阳性率为18%（14/77），CATT阳性率为22%（17/77），实时荧光定量PCR阳性率为60%（46/77），该链式反应检测率分别比其他技术高两倍和三倍。数据分析显示，与实时荧光定量PCR相比，显微镜检查和CATT的相对灵敏度分别为30.4%和37.0%。实时荧光定量PCR与其他方法的灵敏度差异具有统计学意义，X值分别为30.03和20.1（两种情况下自由度均为1，P值均为0.05）。显微镜检查结果与CATT结果一致性良好（κ = 0.72；95%可信区间 = 0.62 - 0.82）。科恩卡帕指数显示实时荧光定量PCR与显微镜检查一致性一般（κ = 0.26；95%可信区间 = 0.16 - 0.36），而与CATT一致性较差（κ = 0.09；95%可信区间 = 0.02 - 0.15）。