Guangdong Key Laboratory of Nanomedicine, CAS Key Laboratory of Health Informatics, Shenzhen Bioactive Materials Engineering Lab for Medicine, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
J Mater Chem B. 2022 Jun 8;10(22):4254-4260. doi: 10.1039/d2tb00422d.
Butyrylcholinesterase (BChE) is an essential human biomarker which is related to liver and neurodegenerative diseases. It is of great significance to develop a fluorescent probe that can image BChE and . Unfortunately, most fluorescent probes that are based on a single change in fluorescence intensity are susceptible to environmental interference. Therefore, we reported an easily available ratiometric fluorescent probe, TB-BChE, with aggregation-induced emission (AIE) characteristics for ratiometric imaging of BChE. TB-BChE demonstrated excellent sensitivity (LOD = 39.24 ng mL) and specificity for BChE. Moreover, we have successfully studied the ratiometric imaging of TB-BChE to BChE in a nonalcoholic fatty liver disease model. These results indicated that TB-BChE is expected to become a powerful analysis tool for butyrylcholinesterase research in basic medicine and clinical applications.
丁酰胆碱酯酶（BChE）是一种重要的人类生物标志物，与肝脏和神经退行性疾病有关。开发一种能够对 BChE 成像的荧光探针具有重要意义。然而，大多数基于荧光强度单一变化的荧光探针容易受到环境干扰。因此，我们报道了一种具有聚集诱导发射（AIE）特性的易得的比率型荧光探针 TB-BChE，用于 BChE 的比率成像。TB-BChE 对 BChE 表现出优异的灵敏度（LOD = 39.24 ng mL）和特异性。此外，我们还成功地研究了 TB-BChE 对非酒精性脂肪性肝病模型中 BChE 的比率成像。这些结果表明，TB-BChE 有望成为基础医学和临床应用中研究丁酰胆碱酯酶的有力分析工具。
