College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Biosensors (Basel). 2022 Nov 1;12(11):953. doi: 10.3390/bios12110953.
Enzymes play a critical role in most complex biochemical processes. Some of them can be regarded as biomarkers for disease diagnosis. Taking advantage of aggregation-induced emission (AIE)-based biosensors, a series of fluorogens with AIE characteristics (AIEgens) have been designed and synthesized for the detection and imaging of enzymes. In this work, we summarized the advances in AIEgens-based probes and sensing platforms for the fluorescent detection of enzymes, including proteases, phosphatases, glycosidases, cholinesterases, telomerase and others. The AIEgens involve organic dyes and metal nanoclusters. This work provides valuable references for the design of novel AIE-based sensing platforms.
酶在大多数复杂的生化过程中起着关键作用。其中一些酶可以作为疾病诊断的生物标志物。利用聚集诱导发光（AIE）生物传感器，设计和合成了一系列具有 AIE 特性的荧光团（AIEgens），用于酶的检测和成像。在这项工作中，我们总结了基于 AIEgens 的探针和传感平台在酶的荧光检测方面的进展，包括蛋白酶、磷酸酶、糖苷酶、胆碱酯酶、端粒酶等。AIEgens 涉及有机染料和金属纳米簇。这项工作为新型基于 AIE 的传感平台的设计提供了有价值的参考。
