监测菌悬液在浸染叶片中的生长情况：“Agromonas”测定法。

Monitoring Pseudomonas syringae Growth in Agroinfiltrated Leaves: The "Agromonas" Assay.

机构信息

Plant Chemetics Lab, Department of Plant Sciences, University of Oxford, Oxford, UK.

出版信息

Methods Mol Biol. 2022;2447:247-259. doi: 10.1007/978-1-0716-2079-3_20.

DOI:10.1007/978-1-0716-2079-3_20

Abstract

We recently developed a simple method called "agromonas" which facilitates the detection of Pseudomonas syringae growth in agroinfiltrated leaves expressing genes of interest. This protocol consists of agroinfiltration followed by inoculation of Pseudomonas syringae by either infiltration or spray inoculation. Next, bacterial growth is measured a few days later by plating leaf extracts out on P. syringae-selective medium. In this protocol, we describe all the steps required for agroinfiltration, inoculation of P. syringae by both injection and spray infection and explain how to quantify in planta live bacteria.

摘要

我们最近开发了一种简单的方法，称为“agromonas”，它可以方便地检测在表达感兴趣基因的农杆菌浸润叶片中假单胞菌生长。该方案包括农杆菌浸润，然后通过浸润或喷雾接种接种假单胞菌。几天后，通过在假单胞菌选择培养基上平板培养叶片提取物来测量细菌生长。在本方案中，我们描述了农杆菌浸润所需的所有步骤，通过注射和喷雾感染接种假单胞菌，并解释了如何定量植物体内活菌。