State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock (R2BGL), College of Life Sciences, Inner Mongolia University, Hohhot, China.
Laboratory of Microbiology and Immunology, College of Basic Medicine, Inner Mongolia Medical University, Hohhot, China.
FASEB J. 2022 Jun;36(6):e22348. doi: 10.1096/fj.202200152R.
Spermatogenesis is a highly coordinated and complex process, and is pivotal for transmitting genetic information between mammalian generations. In this study, we investigated the conservation, differences, and biological functions of homologous genes during spermatogenesis in Mongolia sheep, humans, cynomolgus monkey, and mice using single-cell RNA sequencing technology. We compared X chromosome meiotic inactivation events in Mongolia sheep, humans, cynomolgus monkey, and mice to uncover the concerted activity of X chromosome genes. Subsequently, we focused on the dynamics of gene expression, key biological functions, and signaling pathways at various stages of spermatogenesis in Mongolia sheep and humans. Additionally, the ligand-receptor networks of Mongolia sheep and humans in testicular somatic and germ cells at different developmental stages were mapped to reveal conserved germ cell-soma communication using single-cell resolution. These datasets provided novel information and insights to unravel the molecular regulatory mechanisms of Mongolia sheep spermatogenesis and highlight conservation in gene expression during spermatogenesis between Mongolia sheep and humans, providing a foundation for the establishment of a large mammalian disease model of male infertility.
精子发生是一个高度协调和复杂的过程，对于哺乳动物世代之间遗传信息的传递至关重要。在这项研究中，我们使用单细胞 RNA 测序技术研究了蒙古绵羊、人类、食蟹猴和小鼠精子发生过程中同源基因的保守性、差异和生物学功能。我们比较了蒙古绵羊、人类、食蟹猴和小鼠的 X 染色体减数分裂失活事件，以揭示 X 染色体基因的协同活性。随后，我们专注于蒙古绵羊和人类精子发生各个阶段的基因表达动态、关键生物学功能和信号通路。此外，我们还绘制了蒙古绵羊和人类在睾丸体和生殖细胞不同发育阶段的配体-受体网络，以揭示使用单细胞分辨率的保守生殖细胞-体细胞通讯。这些数据集提供了新的信息和见解，以揭示蒙古绵羊精子发生的分子调控机制，并强调了蒙古绵羊和人类精子发生过程中基因表达的保守性，为建立大型哺乳动物男性不育疾病模型奠定了基础。
