描述来自亚马逊地区的伊蚊属（双翅目：蚊科）的线粒体基因组和系统发育。

Description of the mitogenome and phylogeny of Aedes spp. (Diptera: Culicidae) from the Amazon region.

机构信息

Center of Biological and Health Sciences, State of Pará University, Belém 66095-662, Brazil.

Center of Biological and Health Sciences, State of Pará University, Belém 66095-662, Brazil; Evandro Chagas Institute - IEC/SVS/MS, Department of Arbovirology and Hemorrhagic Fevers, Ananindeua 67030-000, Brazil.

出版信息

Acta Trop. 2022 Aug;232:106500. doi: 10.1016/j.actatropica.2022.106500. Epub 2022 May 16.

DOI:10.1016/j.actatropica.2022.106500

PMID:35584780

Abstract

The genus Aedes (Diptera: Culicidae) includes species of great epidemiological relevance, particularly involved in transmission cycles of leading arboviruses in the Brazilian Amazon region, such as the Zika virus (ZIKV), Dengue virus (DENV), Yellow fever virus (YFV), and Chikungunya virus (CHIKV). We report here the first putatively complete sequencing of the mitochondrial genomes of Brazilian populations of the species Aedes albopictus, Aedes scapularis and Aedes serratus. The sequences obtained showed an average length of 14,947 bp, comprising 37 functional subunits, typical in animal mitochondria (13 PCGs, 22 tRNA, and 2 rRNA). The phylogeny reconstructed by Maximum likelihood method, based on the concatenated sequences of all 13 PCGs produced at least two non-directly related groupings, composed of representatives of the subgenus Ochlerotatus and Stegomyia of the genus Aedes. The data and information produced here may be useful for future taxonomic and evolutionary studies of the genus Aedes, as well as the Culicidae family.

摘要

按蚊属（双翅目：蚊科）包括具有重要流行病学意义的物种，特别是在巴西亚马逊地区主要虫媒病毒的传播循环中，如寨卡病毒（ZIKV）、登革热病毒（DENV）、黄热病病毒（YFV）和基孔肯雅热病毒（CHIKV）。我们在这里报告了巴西白纹伊蚊、埃及伊蚊和三带喙库蚊物种的线粒体基因组的首次推定完整测序。获得的序列平均长度为 14947bp，包含 37 个功能亚基，这是动物线粒体的典型特征（13 个 PCG、22 个 tRNA 和 2 个 rRNA）。基于所有 13 个 PCG 串联序列重建的最大似然法系统发育树产生了至少两个非直接相关的分组，由 Ochlerotatus 亚属和 Aedes 属的 Stegomyia 代表组成。这里产生的数据和信息可能对按蚊属以及蚊科的未来分类学和进化研究有用。