Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia; Grupo de Investigación en Ciencias Básicas (NÚCLEO) Facultad de Ciencias e Ingeniería, Universidad de Boyacá, Tunja, Colombia.
Acta Trop. 2023 Sep;245:106972. doi: 10.1016/j.actatropica.2023.106972. Epub 2023 Jun 16.
Studies focused on identifying the viral species of Flavivirus in vectors are scarce in Latin America and particularly in Colombia. Therefore, the frequency of infection of the Flavivirus genus and its feeding preferences were identified in the mosquito species circulating in the municipality of Puerto Carreño-Vichada, located in the Eastern Plains of Colombia. This was done by sequencing the viral NS5 and vertebrate 12S rRNA genes, respectively, using Oxford Nanopore Technologies (ONT). A total of 1,159 mosquitoes were captured, with the most abundant species being Aedes serratus at 73.6% (n = 853). All the mosquitoes were processed in 230 pools (2-6 individuals) and 51 individuals, where 37.01% (n = 104) were found to be infected with Flavivirus. In these samples, infection by arboviruses of epidemiological importance, such as dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV), was ruled out by PCR. However, through sequencing, infection by different insect-specific viruses (ISFVs) and a medically important virus, West Nile virus (WNV), were identified in a mosquito of the Culex browni species. Additionally, the feeding patterns showed that most species present a generalist behavior. Given the above, conducting entomovirological surveillance studies is crucial, especially in areas of low anthropogenic intervention, due to the high probability that potentially pathogenic viruses could generate spillover events under deforestation scenarios.
针对拉丁美洲，特别是哥伦比亚，在媒介中鉴定黄病毒种方面的研究还很少。因此，本研究通过牛津纳米孔技术（ONT）分别对病毒 NS5 和脊椎动物 12S rRNA 基因进行测序，确定了在哥伦比亚东部平原的波多卡雷尼奥-维查达市循环的蚊子种类中黄病毒属的感染频率及其摄食偏好。共捕获了 1159 只蚊子，其中最丰富的物种是 Aedes serratus，占 73.6%（n=853）。所有蚊子都在 230 个池中（2-6 只个体）和 51 只个体中进行了处理，其中 37.01%（n=104）被发现感染了黄病毒。在这些样本中，通过 PCR 排除了登革热病毒（DENV）、寨卡病毒（ZIKV）和基孔肯雅热病毒（CHIKV）等具有流行病学重要性的虫媒病毒感染。然而，通过测序，在一种属于 Culex browni 种的蚊子中鉴定出了不同昆虫特异性病毒（ISFVs）和一种医学上重要的病毒，即西尼罗河病毒（WNV）。此外，摄食模式表明，大多数物种表现出泛食性。鉴于上述情况，开展昆虫病毒学监测研究至关重要，特别是在人为干预程度较低的地区，因为在森林砍伐情景下，潜在的致病病毒很可能会引发溢出事件。
