Department of Internal Medicine and Hematology, Laboratory of Flow Cytometry, Military Institute of Medicine, Warsaw, Poland.
Department of Internal Medicine, Pulmonology, Allergology and Clinical Immunology, Military Institute of Medicine, Warsaw, Poland.
Front Immunol. 2022 May 2;13:864497. doi: 10.3389/fimmu.2022.864497. eCollection 2022.
Lymphocytes play a leading role in regulation of the immune system in lung cancer patients. The recognition of T cells profile may help in prediction of effectiveness of anticancer immunotherapy. The aim of the study was to determine the dominant subpopulation of CD4+ and CD8+ lymphocytes in metastatic and non-metastatic lymph nodes (LNs) of lung cancer patients. LNs aspirates were obtained during EBUS/TBNA procedure and cells were analyzed by flow cytometry. We showed a higher percentage of CD4+ and CD8+ effector memory T cells in the metastatic than in the non-metastatic LNs (28.6 vs. 15.3% and 28.6 vs. 14.0%, p< 0.05). The proportion of CD45RO+ T regulatory cells (CD45RO+ Tregs) was higher in the metastatic LNs than in the non-metastatic ones (65.6 vs. 31%, p< 0.05). We reported the significant differences in T cell subsets depending on the lung cancer metastatic process. We observed that the effector memory T cells were predominant subpopulations in metastatic LNs. Lymphocyte profile in LNs is easy to evaluate by flow cytometry of EBUS/TBNA samples and may reflect the immune status in lung cancer.
淋巴细胞在肺癌患者的免疫系统调节中发挥主导作用。T 细胞表型的识别可能有助于预测抗癌免疫治疗的效果。本研究的目的是确定转移性和非转移性肺癌患者淋巴结（LNs）中 CD4+和 CD8+淋巴细胞的优势亚群。在 EBUS/TBNA 操作过程中获取 LNs 吸出物，并通过流式细胞术分析细胞。我们发现转移性 LNs 中 CD4+和 CD8+效应记忆 T 细胞的比例高于非转移性 LNs（28.6%比 15.3%和 28.6%比 14.0%，p<0.05）。转移性 LNs 中 CD45RO+调节性 T 细胞（CD45RO+Tregs）的比例高于非转移性 LNs（65.6%比 31%，p<0.05）。我们报告了 T 细胞亚群根据肺癌转移过程的显著差异。我们观察到，效应记忆 T 细胞是转移性 LNs 中的主要亚群。通过 EBUS/TBNA 样本的流式细胞术很容易评估 LNs 中的淋巴细胞谱，并且可能反映肺癌中的免疫状态。
