Houghten R A, DeGraw S T
J Chromatogr. 1987 Jan 16;386:223-8. doi: 10.1016/s0021-9673(01)94599-6.
While attempting to derive a set of high-performance liquid chromatographic amino acid retention coefficients from a set of 298 related peptide analogues of a 13-amino acid peptide, we found that each position within the peptide would require a different set of retention coefficients to accurately predict peptide retention times. Furthermore, our results show that peptides having the same amino composition but slightly different sequences can have very different retention times. We believe that individual sequence domains and the resulting differences in the solid phase-mobile phase interactions must be taken into account for the accurate prediction of peptide retention times.
在尝试从一组由13个氨基酸组成的肽的298个相关肽类似物中推导出一套高效液相色谱氨基酸保留系数时，我们发现肽内的每个位置都需要一套不同的保留系数，才能准确预测肽的保留时间。此外，我们的结果表明，氨基酸组成相同但序列略有不同的肽可能具有非常不同的保留时间。我们认为，为了准确预测肽的保留时间，必须考虑单个序列域以及由此产生的固-液相互作用差异。
