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High-performance liquid chromatographic analysis of amino acids and peptide-hormone hydrolysates in the picomole range.

作者信息

Voelter W, Zech K

出版信息

J Chromatogr. 1975 Oct 29;112:643-9. doi: 10.1016/s0021-9673(00)99993-x.

DOI:10.1016/s0021-9673(00)99993-x
PMID:1184693
Abstract

A high-pressure liquid chromatographic system for the separation of amino acids and peptide-hormone hydrolysates is described. A modified high-pressure liquid chromatography (Hewlett-Packard, model 1010B) equipped with an automatic gradient-generating system, and the applications of fluorescamine and ion-exchange resins of particle size 8 +/- 2 mum permit the detection of amino acids in the picomole range with good resolution. Compared with conventional amino acid analyzers, the times for separation are reduced by a factor of 3--4.

摘要

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